General Stability | Organism |
---|---|
Mg2+ stabilizes | Saccharomycopsis lipolytica |
Mn2+ stabilizes | Saccharomycopsis lipolytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoadipate | - |
Saccharomycopsis lipolytica | |
homoisocitrate | - |
Saccharomycopsis lipolytica | |
Oxaloglutarate | - |
Saccharomycopsis lipolytica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | required for optimal activity | Saccharomycopsis lipolytica | |
Mg2+ | required | Saccharomycopsis lipolytica | |
Mn2+ | stimulation of oxidative decarboxylation | Saccharomycopsis lipolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
native or denaturing PAGE | Saccharomycopsis lipolytica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-hydroxy-1,2,4-butanetricarboxylate + NAD+ | Saccharomycopsis lipolytica | production of 2-oxoadipic acid, a precursor of lysine biosynthesis | 2-oxoadipate + CO2 + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomycopsis lipolytica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomycopsis lipolytica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.304 | - |
- |
Saccharomycopsis lipolytica |
Storage Stability | Organism |
---|---|
-20°C, stable as ammonium sulfate precipitate | Saccharomycopsis lipolytica |
0°C, pH 7.2, rapid loss of activity | Saccharomycopsis lipolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-hydroxy-1,2,4-butanetricarboxylate + NAD+ | - |
Saccharomycopsis lipolytica | 2-oxoadipate + CO2 + NADH | - |
? | |
1-hydroxy-1,2,4-butanetricarboxylate + NAD+ | production of 2-oxoadipic acid, a precursor of lysine biosynthesis | Saccharomycopsis lipolytica | 2-oxoadipate + CO2 + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 48000, at high protein concentration or in presence of substrate the enzyme exists as dimer | Saccharomycopsis lipolytica |
monomer | 1 * 48000, native or denaturing PAGE | Saccharomycopsis lipolytica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Saccharomycopsis lipolytica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Saccharomycopsis lipolytica | |
NADH | - |
Saccharomycopsis lipolytica |