Protein Variants | Comment | Organism |
---|---|---|
G50D/S52D | mutant engineered for reversal in cofactor specificity for NADH over NADPH | Methanococcus aeolicus |
R48P/S51L/S52D | mutant engineered for reversal in cofactor specificity for NADH over NADPH | Alicyclobacillus acidocaldarius subsp. acidocaldarius |
R48P/S51L/S52D/R84A | mutant engineered for reversal in cofactor specificity for NADH over NADPH | Alicyclobacillus acidocaldarius subsp. acidocaldarius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0173 | - |
NADPH | wild-type, pH not specified in the publication, temperature not specified in the publication | Methanococcus aeolicus | |
0.018 | - |
NADPH | wild-type, pH not specified in the publication, temperature not specified in the publication | Alicyclobacillus acidocaldarius subsp. acidocaldarius | |
0.026 | - |
NADH | mutant G50D/S52D, pH not specified in the publication, temperature not specified in the publication | Methanococcus aeolicus | |
0.027 | - |
NADH | mutant R48P/S51L/S52D/R84A, pH not specified in the publication, temperature not specified in the publication | Alicyclobacillus acidocaldarius subsp. acidocaldarius | |
0.028 | - |
NADH | wild-type, pH not specified in the publication, temperature not specified in the publication | Alicyclobacillus acidocaldarius subsp. acidocaldarius | |
0.043 | - |
NADH | mutant R48P/S51L/S52D, pH not specified in the publication, temperature not specified in the publication | Alicyclobacillus acidocaldarius subsp. acidocaldarius | |
0.059 | - |
NADH | wild-type, pH not specified in the publication, temperature not specified in the publication | Methanococcus aeolicus | |
0.08 | - |
NADPH | mutant G50D/S52D, pH not specified in the publication, temperature not specified in the publication | Methanococcus aeolicus | |
1 | - |
NADPH | mutant R48P/S51L/S52D, pH not specified in the publication, temperature not specified in the publication | Alicyclobacillus acidocaldarius subsp. acidocaldarius | |
1 | - |
NADPH | mutant R48P/S51L/S52D/R84A, pH not specified in the publication, temperature not specified in the publication | Alicyclobacillus acidocaldarius subsp. acidocaldarius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alicyclobacillus acidocaldarius subsp. acidocaldarius | C8WR67 | - |
- |
Methanococcus aeolicus | A6UW80 | - |
- |
Methanococcus aeolicus DSM 17508 | A6UW80 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-acetolactate + NADH + H+ | - |
Alicyclobacillus acidocaldarius subsp. acidocaldarius | 2,3-dihydroxy-3-methylbutanoate + NAD+ | - |
? | |
(S)-2-acetolactate + NADH + H+ | - |
Methanococcus aeolicus | 2,3-dihydroxy-3-methylbutanoate + NAD+ | - |
? | |
(S)-2-acetolactate + NADH + H+ | - |
Methanococcus aeolicus DSM 17508 | 2,3-dihydroxy-3-methylbutanoate + NAD+ | - |
? | |
(S)-2-acetolactate + NADPH + H+ | - |
Alicyclobacillus acidocaldarius subsp. acidocaldarius | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
? | |
(S)-2-acetolactate + NADPH + H+ | - |
Methanococcus aeolicus | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
? | |
(S)-2-acetolactate + NADPH + H+ | - |
Methanococcus aeolicus DSM 17508 | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ilvC | - |
Alicyclobacillus acidocaldarius subsp. acidocaldarius |
ilvC | - |
Methanococcus aeolicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
NADPH | mutant G50D/S52D, pH not specified in the publication, temperature not specified in the publication | Methanococcus aeolicus | |
0.01 | - |
NADPH | mutant R48P/S51L/S52D/R84A, pH not specified in the publication, temperature not specified in the publication | Alicyclobacillus acidocaldarius subsp. acidocaldarius | |
0.013 | - |
NADPH | mutant R48P/S51L/S52D, pH not specified in the publication, temperature not specified in the publication | Alicyclobacillus acidocaldarius subsp. acidocaldarius | |
0.03 | - |
NADH | mutant R48P/S51L/S52D/R84A, pH not specified in the publication, temperature not specified in the publication | Alicyclobacillus acidocaldarius subsp. acidocaldarius | |
0.032 | - |
NADH | mutant R48P/S51L/S52D, pH not specified in the publication, temperature not specified in the publication | Alicyclobacillus acidocaldarius subsp. acidocaldarius | |
0.15 | - |
NADH | mutant G50D/S52D, pH not specified in the publication, temperature not specified in the publication | Methanococcus aeolicus | |
0.26 | - |
NADH | wild-type, pH not specified in the publication, temperature not specified in the publication | Alicyclobacillus acidocaldarius subsp. acidocaldarius | |
0.3 | - |
NADH | wild-type, pH not specified in the publication, temperature not specified in the publication | Methanococcus aeolicus | |
0.66 | - |
NADPH | wild-type, pH not specified in the publication, temperature not specified in the publication | Alicyclobacillus acidocaldarius subsp. acidocaldarius | |
0.7 | - |
NADPH | wild-type, pH not specified in the publication, temperature not specified in the publication | Methanococcus aeolicus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0046 | - |
NADH | wild-type, pH not specified in the publication, temperature not specified in the publication | Methanococcus aeolicus | |
0.0062 | - |
NADH | mutant G50D/S52D, pH not specified in the publication, temperature not specified in the publication | Methanococcus aeolicus | |
0.01 | - |
NADPH | mutant R48P/S51L/S52D/R84A, pH not specified in the publication, temperature not specified in the publication | Alicyclobacillus acidocaldarius subsp. acidocaldarius | |
0.013 | - |
NADPH | mutant R48P/S51L/S52D, pH not specified in the publication, temperature not specified in the publication | Alicyclobacillus acidocaldarius subsp. acidocaldarius | |
0.043 | - |
NADPH | wild-type, pH not specified in the publication, temperature not specified in the publication | Methanococcus aeolicus | |
0.05 | - |
NADPH | mutant G50D/S52D, pH not specified in the publication, temperature not specified in the publication | Methanococcus aeolicus | |
0.7 | - |
NADH | mutant R48P/S51L/S52D, pH not specified in the publication, temperature not specified in the publication | Alicyclobacillus acidocaldarius subsp. acidocaldarius | |
1.1 | - |
NADH | mutant R48P/S51L/S52D/R84A, pH not specified in the publication, temperature not specified in the publication | Alicyclobacillus acidocaldarius subsp. acidocaldarius | |
9 | - |
NADH | wild-type, pH not specified in the publication, temperature not specified in the publication | Alicyclobacillus acidocaldarius subsp. acidocaldarius | |
37 | - |
NADPH | wild-type, pH not specified in the publication, temperature not specified in the publication | Alicyclobacillus acidocaldarius subsp. acidocaldarius |