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Literature summary for 1.1.1.86 extracted from

  • Brinkmann-Chen, S.; Flock, T.; Cahn, J.; Snow, C.; Brustad, E.; McIntosh, J.; Meinhold, P.; Zhang, L.; Arnold, F.
    General approach to reversing ketol-acid reductoisomerase cofactor dependence from NADPH to NADH (2013), Proc. Natl. Acad. Sci. USA, 110, 10946-10951.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
G50D/S52D mutant engineered for reversal in cofactor specificity for NADH over NADPH Methanococcus aeolicus
R48P/S51L/S52D mutant engineered for reversal in cofactor specificity for NADH over NADPH Alicyclobacillus acidocaldarius subsp. acidocaldarius
R48P/S51L/S52D/R84A mutant engineered for reversal in cofactor specificity for NADH over NADPH Alicyclobacillus acidocaldarius subsp. acidocaldarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0173
-
NADPH wild-type, pH not specified in the publication, temperature not specified in the publication Methanococcus aeolicus
0.018
-
NADPH wild-type, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
0.026
-
NADH mutant G50D/S52D, pH not specified in the publication, temperature not specified in the publication Methanococcus aeolicus
0.027
-
NADH mutant R48P/S51L/S52D/R84A, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
0.028
-
NADH wild-type, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
0.043
-
NADH mutant R48P/S51L/S52D, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
0.059
-
NADH wild-type, pH not specified in the publication, temperature not specified in the publication Methanococcus aeolicus
0.08
-
NADPH mutant G50D/S52D, pH not specified in the publication, temperature not specified in the publication Methanococcus aeolicus
1
-
NADPH mutant R48P/S51L/S52D, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
1
-
NADPH mutant R48P/S51L/S52D/R84A, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius

Organism

Organism UniProt Comment Textmining
Alicyclobacillus acidocaldarius subsp. acidocaldarius C8WR67
-
-
Methanococcus aeolicus A6UW80
-
-
Methanococcus aeolicus DSM 17508 A6UW80
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-acetolactate + NADH + H+
-
Alicyclobacillus acidocaldarius subsp. acidocaldarius 2,3-dihydroxy-3-methylbutanoate + NAD+
-
?
(S)-2-acetolactate + NADH + H+
-
Methanococcus aeolicus 2,3-dihydroxy-3-methylbutanoate + NAD+
-
?
(S)-2-acetolactate + NADH + H+
-
Methanococcus aeolicus DSM 17508 2,3-dihydroxy-3-methylbutanoate + NAD+
-
?
(S)-2-acetolactate + NADPH + H+
-
Alicyclobacillus acidocaldarius subsp. acidocaldarius 2,3-dihydroxy-3-methylbutanoate + NADP+
-
?
(S)-2-acetolactate + NADPH + H+
-
Methanococcus aeolicus 2,3-dihydroxy-3-methylbutanoate + NADP+
-
?
(S)-2-acetolactate + NADPH + H+
-
Methanococcus aeolicus DSM 17508 2,3-dihydroxy-3-methylbutanoate + NADP+
-
?

Synonyms

Synonyms Comment Organism
ilvC
-
Alicyclobacillus acidocaldarius subsp. acidocaldarius
ilvC
-
Methanococcus aeolicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.004
-
NADPH mutant G50D/S52D, pH not specified in the publication, temperature not specified in the publication Methanococcus aeolicus
0.01
-
NADPH mutant R48P/S51L/S52D/R84A, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
0.013
-
NADPH mutant R48P/S51L/S52D, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
0.03
-
NADH mutant R48P/S51L/S52D/R84A, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
0.032
-
NADH mutant R48P/S51L/S52D, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
0.15
-
NADH mutant G50D/S52D, pH not specified in the publication, temperature not specified in the publication Methanococcus aeolicus
0.26
-
NADH wild-type, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
0.3
-
NADH wild-type, pH not specified in the publication, temperature not specified in the publication Methanococcus aeolicus
0.66
-
NADPH wild-type, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
0.7
-
NADPH wild-type, pH not specified in the publication, temperature not specified in the publication Methanococcus aeolicus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0046
-
NADH wild-type, pH not specified in the publication, temperature not specified in the publication Methanococcus aeolicus
0.0062
-
NADH mutant G50D/S52D, pH not specified in the publication, temperature not specified in the publication Methanococcus aeolicus
0.01
-
NADPH mutant R48P/S51L/S52D/R84A, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
0.013
-
NADPH mutant R48P/S51L/S52D, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
0.043
-
NADPH wild-type, pH not specified in the publication, temperature not specified in the publication Methanococcus aeolicus
0.05
-
NADPH mutant G50D/S52D, pH not specified in the publication, temperature not specified in the publication Methanococcus aeolicus
0.7
-
NADH mutant R48P/S51L/S52D, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
1.1
-
NADH mutant R48P/S51L/S52D/R84A, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
9
-
NADH wild-type, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
37
-
NADPH wild-type, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius