Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
native enzyme with two magnesium ions bound in the active site, hanging drop vapor diffusion method, using 250 mM MgCl2, 20% (w/v) PEG 3350, 100 mM Tris/HCl, pH 8.0 | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-Hydroxy-N-isopropyloxamate | - |
Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
(S)-2-acetolactate | at pH 8.5 and 37°C | Mycobacterium tuberculosis | |
2.1 | - |
3-hydroxypyruvate | at pH 8.5 and 37°C | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
multi-angle light scattering | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-acetolactate + NADPH + H+ | Mycobacterium tuberculosis | - |
(R)-2,3-dihydroxyisovalerate + NADP+ | - |
r | |
(S)-2-acetolactate + NADPH + H+ | Mycobacterium tuberculosis ATCC 25618 | - |
(R)-2,3-dihydroxyisovalerate + NADP+ | - |
r | |
2-aceto-2-hydroxybutyrate + NADPH + H+ | Mycobacterium tuberculosis | - |
? + NADP+ | - |
r | |
2-aceto-2-hydroxybutyrate + NADPH + H+ | Mycobacterium tuberculosis ATCC 25618 | - |
? + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WKJ7 | - |
- |
Mycobacterium tuberculosis ATCC 25618 | P9WKJ7 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA-agarose resin column chromatography and Superdex 200 gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-acetolactate + NADPH + H+ | - |
Mycobacterium tuberculosis | (R)-2,3-dihydroxyisovalerate + NADP+ | - |
r | |
(S)-2-acetolactate + NADPH + H+ | - |
Mycobacterium tuberculosis ATCC 25618 | (R)-2,3-dihydroxyisovalerate + NADP+ | - |
r | |
2-aceto-2-hydroxybutyrate + NADPH + H+ | - |
Mycobacterium tuberculosis | ? + NADP+ | - |
r | |
2-aceto-2-hydroxybutyrate + NADPH + H+ | - |
Mycobacterium tuberculosis ATCC 25618 | ? + NADP+ | - |
r | |
3-hydroxypyruvate + NADPH + H+ | - |
Mycobacterium tuberculosis | ? + NADP+ | - |
r | |
3-hydroxypyruvate + NADPH + H+ | - |
Mycobacterium tuberculosis ATCC 25618 | ? + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 37155, mass spectrometry | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
KARI | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
(S)-2-acetolactate | at pH 8.5 and 37°C | Mycobacterium tuberculosis | |
12.5 | - |
3-hydroxypyruvate | at pH 8.5 and 37°C | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000974 | - |
N-Hydroxy-N-isopropyloxamate | at pH 8.5 and 37°C | Mycobacterium tuberculosis |