Literature summary for 1.1.1.86 extracted from

Dumas, R.; Biou, V.; Halgand, F.; Douce, R.; Duggleby, R.G.
Enzymology, structure, and dynamics of acetohydroxy acid isomeroreductase (2001), Acc. Chem. Res., 34, 399-408.

Search for more literature for 1.1.1.86

Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Spinacia oleracea

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Escherichia coli
Mg2+	required	Spinacia oleracea
Mg2+	two ions bind to enzyme	Spinacia oleracea
additional information	no metal ions required	Triticum aestivum
additional information	Mn2+, Co2+, Ni2+, Zn2+, Ca2+, Cu2+ and Co3+ can not substitute for Mg2+	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-acetolactate + NADPH	Escherichia coli	enzyme of branched chain amino acid synthesis	2,3-dihydroxy-3-methylbutanoate + NADP+	-	r
2-acetolactate + NADPH	Triticum aestivum	enzyme of branched chain amino acid synthesis	2,3-dihydroxy-3-methylbutanoate + NADP+	-	r
2-acetolactate + NADPH	Spinacia oleracea	enzyme of branched chain amino acid synthesis	2,3-dihydroxy-3-methylbutanoate + NADP+	-	r

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
Spinacia oleracea	Q01292	-	-
Triticum aestivum	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-acetolactate + NADPH	enzyme of branched chain amino acid synthesis	Escherichia coli	2,3-dihydroxy-3-methylbutanoate + NADP+	-	r
2-acetolactate + NADPH	enzyme of branched chain amino acid synthesis	Triticum aestivum	2,3-dihydroxy-3-methylbutanoate + NADP+	-	r
2-acetolactate + NADPH	enzyme of branched chain amino acid synthesis	Spinacia oleracea	2,3-dihydroxy-3-methylbutanoate + NADP+	-	r
NADPH + 2-acetolactate	-	Escherichia coli	NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate	-	?
NADPH + 2-acetolactate	-	Triticum aestivum	NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate	-	?
NADPH + 2-acetolactate	-	Spinacia oleracea	NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate	-	?

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Escherichia coli
NADPH	-	Triticum aestivum
NADPH	-	Spinacia oleracea

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Release 2023.1 (January 2023)