Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Spinacia oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-dihydroxy-3-isovalerate | - |
Spinacia oleracea | |
2-hydroxy-2-methyl-3-oxopentanoate | - |
Spinacia oleracea | |
NADP+ | product inhibition | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
NADPH | pH 8.2, 30°C | Spinacia oleracea | |
0.01 | - |
2-acetolactate | pH 8.2, 30°C | Spinacia oleracea | |
0.01 | - |
2-aceto-2-hydroxybutyrate | pH 8.2, 30°C | Spinacia oleracea | |
0.101 | - |
NADH | pH 8.2, 30°C | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Spinacia oleracea | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Spinacia oleracea | |
Mg2+ | Km for Mg2+: 0.0045 mM, at pH 8.2, 30°C | Spinacia oleracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56850 | - |
2 * 56850, calculation from acid sequence | Spinacia oleracea |
57000 | - |
2 * 57000, SDS-PAGE | Spinacia oleracea |
115000 | - |
recombinant enzyme expressed in E. coli, gel filtration | Spinacia oleracea |
205000 | - |
non-denaturing SDS-PAGE | Spinacia oleracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-acetolactate + NADPH | Spinacia oleracea | enzyme of branched chain amino acid synthesis | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
overexpressed in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli | Spinacia oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5 | - |
- |
Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-acetolactate + NADH + H+ | - |
Spinacia oleracea | 2,3-dihydroxy-3-methylbutanoate + NAD+ + H+ | - |
r | |
2-acetolactate + NADPH | enzyme of branched chain amino acid synthesis | Spinacia oleracea | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
NADPH + 2-aceto-2-hydroxybutyrate | - |
Spinacia oleracea | NADP+ + ? | - |
? | |
NADPH + 2-acetolactate | - |
Spinacia oleracea | NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 57000, SDS-PAGE | Spinacia oleracea |
dimer | 2 * 56850, calculation from acid sequence | Spinacia oleracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Spinacia oleracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Spinacia oleracea |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
NADP+ | pH 8.2, 30°C, NADPH as variable substrate, 0.2 mM (2S)-2-acetolactate as fixed substrate | Spinacia oleracea | |
0.0075 | - |
NADP+ | pH 8.2, 30°C, NADPH as variable substrate, 0.2 mM (2S)-2-aceto-2-hydroxybutanoate as fixed substrate | Spinacia oleracea | |
0.095 | - |
2,3-dihydroxyisovalerate | pH 8.2, 30°C, (2S)-2-acetolactate as variable substrate, 0.250 mM NADPH as fixed substrate | Spinacia oleracea | |
0.145 | - |
2,3-dihydroxy-3-isovalerate | pH 8.2, 30°C, (2S)-2-aceto-2-hydroxybutanoate as variable substrate, 0.250 mM NADPH as fixed substrate | Spinacia oleracea | |
0.225 | - |
NADP+ | pH 8.2, 30°C, (2S)-2-aceto-2-hydroxybutanoate as variable substrate, 0.075 mM NADPH as fixed substrate | Spinacia oleracea | |
0.24 | - |
2-hydroxy-2-methyl-3-oxopentanoate | pH 8.2, 30°C, (2S)-2-aceto-2-hydroxybutanoate as variable substrate, 0.075 mM NADPH as fixed substrate | Spinacia oleracea | |
0.245 | - |
2-hydroxy-2-methyl-3-oxopentanoate | pH 8.2, 30°C, (2S)-2-acetolactate as variable substrate, 0.075 mM NADPH as fixed substrate | Spinacia oleracea | |
0.33 | - |
NADP+ | pH 8.2, 30°C, (2S)-2-acetolactate as variable substrate, 0.075 mM NADPH as fixed substrate | Spinacia oleracea | |
0.935 | - |
2,3-dihydroxyisovalerate | pH 8.2, 30°C, NADPH as variable substrate, 0.2 mM (2S)-2-acetolactate as fixed substrate | Spinacia oleracea | |
1.5 | - |
2,3-dihydroxyisovalerate | pH 8.2, 30°C, NADPH as variable substrate, 0.2 mM (2S)-2-aceto-2-hydroxybutanoate as fixed substrate | Spinacia oleracea | |
1.7 | - |
2-hydroxy-2-methyl-3-oxopentanoate | pH 8.2, 30°C, NADPH as variable substrate, 0.2 mM (2S)-2-acetolactate as fixed substrate | Spinacia oleracea | |
3.6 | - |
2-hydroxy-2-methyl-3-oxopentanoate | pH 8.2, 30°C, NADPH as variable substrate, 0.2 mM (2S)-2-aceto-2-hydroxybutanoate as fixed substrate | Spinacia oleracea |