Literature summary for 1.1.1.86 extracted from

Aulabaugh, A.; Schloss, J.V.
Oxalyl hydroxamates as reaction-intermediate analogues for ketol-acid reductoisomerase (1990), Biochemistry, 29, 2824-2830.

Search for more literature for 1.1.1.86

Inhibitors

Inhibitors	Comment	Organism	Structure
N-Hydroxy-N-isopropyloxamate	very strong, nearly irreversible inhibition, inhibition also with N-alkyl substituted derivates, tightest binding in presence of Mg2+ and NADPH	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.002	-	NADPH	pH 7.4, 25°C	Escherichia coli
0.002	-	2-aceto-2-hydroxybutyrate	pH 7.4, 25°C	Escherichia coli
0.014	-	2-acetolactate	pH 7.4, 25°C	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
55000	-	x * 55000, SDS-PAGE	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-acetolactate + NADPH	Escherichia coli	enzyme of branched chain amino acid synthesis	2,3-dihydroxy-3-methylbutanoate + NADP+	-	r

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.9	-	purified enzyme	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-acetolactate + NADPH	enzyme of branched chain amino acid synthesis	Escherichia coli	2,3-dihydroxy-3-methylbutanoate + NADP+	-	r
acetolactate + NADPH + H+	-	Escherichia coli	2,3-dihydroxy-2-methylbutanoate + NADP+	-	r
NADP+ + 3-hydroxy-3-methyl-2-oxobutanoate	-	Escherichia coli	NADPH + acetolactate	-	?
NADPH + 2-acetolactate	-	Escherichia coli	NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate	-	r

Subunits

Subunits	Comment	Organism
?	x * 55000, SDS-PAGE	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.00417	-	2,3-Dihydroxy-3-methylbutanoate	pH 7.4, 25°C	Escherichia coli
1.8	-	2-acetolactate	pH 7.4, 25°C	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Escherichia coli
NADPH	-	Escherichia coli

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Release 2023.1 (January 2023)