Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbic acid | 5 mM, enhances activity 2-hydroxy-2-ethyl-3-oxobutanoate as substrate | Vigna radiata var. radiata |
General Stability | Organism |
---|---|
high activity loss during DEAE-cellulose chromatography, stable towards acetone fractionation and dialysis, unstable towards freezing and thawing | Vigna radiata var. radiata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arsenite | slight inhibition | Vigna radiata var. radiata | |
ascorbic acid | slight inhibition with 2-acetolactate as substrate | Vigna radiata var. radiata | |
L-ascorbic acid | slightly inhibitory with alpha-acetolactate as substrate | Vigna radiata var. radiata | |
additional information | no inhibition with p-mercuribenzoate, cysteine, 2-mercaptoethanol | Vigna radiata var. radiata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
2-aceto-2-hydroxybutyrate | - |
Vigna radiata var. radiata | |
5.5 | - |
2-acetolactate | - |
Vigna radiata var. radiata |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metal ions required | Vigna radiata var. radiata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-acetolactate + NADPH | Vigna radiata var. radiata | enzyme of branched chain amino acid synthesis | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vigna radiata var. radiata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Vigna radiata var. radiata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-acetolactate + NADPH | enzyme of branched chain amino acid synthesis | Vigna radiata var. radiata | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
NADPH + 2-aceto-2-hydroxybutyrate | - |
Vigna radiata var. radiata | NADP+ + ? | - |
? | |
NADPH + 2-acetolactate | - |
Vigna radiata var. radiata | NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | constant activity with 2-acetolactate as substrate | Vigna radiata var. radiata |
8.6 | - |
with 2-hydroxy-2-ethyl-3-oxobutanoate as substrate, Tris-HCl buffer, alpha-aceto-alpha-hydroxybutyrate as substrate, Tris buffer | Vigna radiata var. radiata |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | pH 7.0: about 40% of maximal activity, pH 9.0: about 90% of maximal activity, with 2-hydroxy-2-ethyl-3-oxobutanoate as substrate, Tris-HCl buffer | Vigna radiata var. radiata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Vigna radiata var. radiata |