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Literature summary for 1.1.1.85 extracted from

  • Kasahara, R.; Sato, T.; Tamegai, H.; Kato, C.
    Piezo-adapted (2R,3S)-3-isopropylmalate dehydrogenase of the obligate piezophile Shewanella benthica DB21MT-2 isolated from the 11,000-m depth of the Mariana Trench (2009), Biosci. Biotechnol. Biochem., 73, 2541-2543.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Shewanella oneidensis
expressed in Escherichia coli as a His-tagged fusion protein Shewanella benthica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0087
-
(2R,3S)-3-isopropylmalate pressure 100 MPa, 20°C, pH not specified in the publication Shewanella oneidensis
0.015
-
(2R,3S)-3-isopropylmalate pressure 0.1 MPa, 20°C, pH not specified in the publication Shewanella oneidensis
0.02
-
(2R,3S)-3-isopropylmalate pressure 100 MPa, 20°C, pH not specified in the publication Shewanella benthica
0.025
-
(2R,3S)-3-isopropylmalate pressure 0.1 MPa, 20°C, pH not specified in the publication Shewanella benthica
0.027
-
(2R,3S)-3-isopropylmalate pressure 50 MPa, 20°C, pH not specified in the publication Shewanella oneidensis
0.032
-
(2R,3S)-3-isopropylmalate pressure 150 MPa, 20°C, pH not specified in the publication Shewanella benthica
0.038
-
(2R,3S)-3-isopropylmalate pressure 200 MPa, 20°C, pH not specified in the publication Shewanella benthica
0.043
-
(2R,3S)-3-isopropylmalate pressure 50 MPa, 20°C, pH not specified in the publication Shewanella benthica
0.053
-
(2R,3S)-3-isopropylmalate pressure 150 MPa, 20°C, pH not specified in the publication Shewanella oneidensis
0.055
-
(2R,3S)-3-isopropylmalate pressure 200 MPa, 20°C, pH not specified in the publication Shewanella oneidensis
0.092
-
(2R,3S)-3-isopropylmalate pressure 250 MPa, 20°C, pH not specified in the publication Shewanella oneidensis
0.108
-
(2R,3S)-3-isopropylmalate pressure 250 MPa, 20°C, pH not specified in the publication Shewanella benthica

Organism

Organism UniProt Comment Textmining
Shewanella benthica D2YZL2
-
-
Shewanella oneidensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using a HisTrap HP column Shewanella oneidensis
using a HisTrap HP column Shewanella benthica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-3-isopropylmalate + NAD+
-
Shewanella oneidensis (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
?
(2R,3S)-3-isopropylmalate + NAD+
-
Shewanella benthica (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
SbIPMDH
-
Shewanella benthica
SoIPMDH
-
Shewanella oneidensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
assay at Shewanella oneidensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1
-
(2R,3S)-3-isopropylmalate pressure 250 MPa, 20°C, pH not specified in the publication Shewanella oneidensis
5.8
-
(2R,3S)-3-isopropylmalate pressure 200 MPa, 20°C, pH not specified in the publication Shewanella oneidensis
7.6
-
(2R,3S)-3-isopropylmalate pressure 100 MPa, 20°C, pH not specified in the publication Shewanella oneidensis
8.4
-
(2R,3S)-3-isopropylmalate pressure 150 MPa, 20°C, pH not specified in the publication Shewanella oneidensis
10
-
(2R,3S)-3-isopropylmalate pressure 250 MPa, 20°C, pH not specified in the publication Shewanella benthica
13
-
(2R,3S)-3-isopropylmalate pressure 200 MPa, 20°C, pH not specified in the publication Shewanella benthica
14
-
(2R,3S)-3-isopropylmalate pressure 0.1 MPa, 20°C, pH not specified in the publication Shewanella oneidensis
14
-
(2R,3S)-3-isopropylmalate pressure 100 MPa, 20°C, pH not specified in the publication Shewanella benthica
14
-
(2R,3S)-3-isopropylmalate pressure 150 MPa, 20°C, pH not specified in the publication Shewanella benthica
15
-
(2R,3S)-3-isopropylmalate pressure 0.1 MPa, 20°C, pH not specified in the publication Shewanella benthica
15
-
(2R,3S)-3-isopropylmalate pressure 50 MPa, 20°C, pH not specified in the publication Shewanella oneidensis
17
-
(2R,3S)-3-isopropylmalate pressure 50 MPa, 20°C, pH not specified in the publication Shewanella benthica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
34
-
(2R,3S)-3-isopropylmalate pressure 250 MPa, 20°C, pH not specified in the publication Shewanella oneidensis
93
-
(2R,3S)-3-isopropylmalate pressure 250 MPa, 20°C, pH not specified in the publication Shewanella benthica
110
-
(2R,3S)-3-isopropylmalate pressure 200 MPa, 20°C, pH not specified in the publication Shewanella oneidensis
160
-
(2R,3S)-3-isopropylmalate pressure 150 MPa, 20°C, pH not specified in the publication Shewanella oneidensis
340
-
(2R,3S)-3-isopropylmalate pressure 200 MPa, 20°C, pH not specified in the publication Shewanella benthica
400
-
(2R,3S)-3-isopropylmalate pressure 50 MPa, 20°C, pH not specified in the publication Shewanella benthica
440
-
(2R,3S)-3-isopropylmalate pressure 150 MPa, 20°C, pH not specified in the publication Shewanella benthica
560
-
(2R,3S)-3-isopropylmalate pressure 50 MPa, 20°C, pH not specified in the publication Shewanella oneidensis
600
-
(2R,3S)-3-isopropylmalate pressure 0.1 MPa, 20°C, pH not specified in the publication Shewanella benthica
700
-
(2R,3S)-3-isopropylmalate pressure 100 MPa, 20°C, pH not specified in the publication Shewanella benthica
870
-
(2R,3S)-3-isopropylmalate pressure 100 MPa, 20°C, pH not specified in the publication Shewanella oneidensis
930
-
(2R,3S)-3-isopropylmalate pressure 0.1 MPa, 20°C, pH not specified in the publication Shewanella oneidensis