Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Shewanella oneidensis |
expressed in Escherichia coli as a His-tagged fusion protein | Shewanella benthica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0087 | - |
(2R,3S)-3-isopropylmalate | pressure 100 MPa, 20°C, pH not specified in the publication | Shewanella oneidensis | |
0.015 | - |
(2R,3S)-3-isopropylmalate | pressure 0.1 MPa, 20°C, pH not specified in the publication | Shewanella oneidensis | |
0.02 | - |
(2R,3S)-3-isopropylmalate | pressure 100 MPa, 20°C, pH not specified in the publication | Shewanella benthica | |
0.025 | - |
(2R,3S)-3-isopropylmalate | pressure 0.1 MPa, 20°C, pH not specified in the publication | Shewanella benthica | |
0.027 | - |
(2R,3S)-3-isopropylmalate | pressure 50 MPa, 20°C, pH not specified in the publication | Shewanella oneidensis | |
0.032 | - |
(2R,3S)-3-isopropylmalate | pressure 150 MPa, 20°C, pH not specified in the publication | Shewanella benthica | |
0.038 | - |
(2R,3S)-3-isopropylmalate | pressure 200 MPa, 20°C, pH not specified in the publication | Shewanella benthica | |
0.043 | - |
(2R,3S)-3-isopropylmalate | pressure 50 MPa, 20°C, pH not specified in the publication | Shewanella benthica | |
0.053 | - |
(2R,3S)-3-isopropylmalate | pressure 150 MPa, 20°C, pH not specified in the publication | Shewanella oneidensis | |
0.055 | - |
(2R,3S)-3-isopropylmalate | pressure 200 MPa, 20°C, pH not specified in the publication | Shewanella oneidensis | |
0.092 | - |
(2R,3S)-3-isopropylmalate | pressure 250 MPa, 20°C, pH not specified in the publication | Shewanella oneidensis | |
0.108 | - |
(2R,3S)-3-isopropylmalate | pressure 250 MPa, 20°C, pH not specified in the publication | Shewanella benthica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shewanella benthica | D2YZL2 | - |
- |
Shewanella oneidensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using a HisTrap HP column | Shewanella oneidensis |
using a HisTrap HP column | Shewanella benthica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ | - |
Shewanella oneidensis | (2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
? | |
(2R,3S)-3-isopropylmalate + NAD+ | - |
Shewanella benthica | (2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SbIPMDH | - |
Shewanella benthica |
SoIPMDH | - |
Shewanella oneidensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Shewanella oneidensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.1 | - |
(2R,3S)-3-isopropylmalate | pressure 250 MPa, 20°C, pH not specified in the publication | Shewanella oneidensis | |
5.8 | - |
(2R,3S)-3-isopropylmalate | pressure 200 MPa, 20°C, pH not specified in the publication | Shewanella oneidensis | |
7.6 | - |
(2R,3S)-3-isopropylmalate | pressure 100 MPa, 20°C, pH not specified in the publication | Shewanella oneidensis | |
8.4 | - |
(2R,3S)-3-isopropylmalate | pressure 150 MPa, 20°C, pH not specified in the publication | Shewanella oneidensis | |
10 | - |
(2R,3S)-3-isopropylmalate | pressure 250 MPa, 20°C, pH not specified in the publication | Shewanella benthica | |
13 | - |
(2R,3S)-3-isopropylmalate | pressure 200 MPa, 20°C, pH not specified in the publication | Shewanella benthica | |
14 | - |
(2R,3S)-3-isopropylmalate | pressure 0.1 MPa, 20°C, pH not specified in the publication | Shewanella oneidensis | |
14 | - |
(2R,3S)-3-isopropylmalate | pressure 100 MPa, 20°C, pH not specified in the publication | Shewanella benthica | |
14 | - |
(2R,3S)-3-isopropylmalate | pressure 150 MPa, 20°C, pH not specified in the publication | Shewanella benthica | |
15 | - |
(2R,3S)-3-isopropylmalate | pressure 0.1 MPa, 20°C, pH not specified in the publication | Shewanella benthica | |
15 | - |
(2R,3S)-3-isopropylmalate | pressure 50 MPa, 20°C, pH not specified in the publication | Shewanella oneidensis | |
17 | - |
(2R,3S)-3-isopropylmalate | pressure 50 MPa, 20°C, pH not specified in the publication | Shewanella benthica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
34 | - |
(2R,3S)-3-isopropylmalate | pressure 250 MPa, 20°C, pH not specified in the publication | Shewanella oneidensis | |
93 | - |
(2R,3S)-3-isopropylmalate | pressure 250 MPa, 20°C, pH not specified in the publication | Shewanella benthica | |
110 | - |
(2R,3S)-3-isopropylmalate | pressure 200 MPa, 20°C, pH not specified in the publication | Shewanella oneidensis | |
160 | - |
(2R,3S)-3-isopropylmalate | pressure 150 MPa, 20°C, pH not specified in the publication | Shewanella oneidensis | |
340 | - |
(2R,3S)-3-isopropylmalate | pressure 200 MPa, 20°C, pH not specified in the publication | Shewanella benthica | |
400 | - |
(2R,3S)-3-isopropylmalate | pressure 50 MPa, 20°C, pH not specified in the publication | Shewanella benthica | |
440 | - |
(2R,3S)-3-isopropylmalate | pressure 150 MPa, 20°C, pH not specified in the publication | Shewanella benthica | |
560 | - |
(2R,3S)-3-isopropylmalate | pressure 50 MPa, 20°C, pH not specified in the publication | Shewanella oneidensis | |
600 | - |
(2R,3S)-3-isopropylmalate | pressure 0.1 MPa, 20°C, pH not specified in the publication | Shewanella benthica | |
700 | - |
(2R,3S)-3-isopropylmalate | pressure 100 MPa, 20°C, pH not specified in the publication | Shewanella benthica | |
870 | - |
(2R,3S)-3-isopropylmalate | pressure 100 MPa, 20°C, pH not specified in the publication | Shewanella oneidensis | |
930 | - |
(2R,3S)-3-isopropylmalate | pressure 0.1 MPa, 20°C, pH not specified in the publication | Shewanella oneidensis |