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Literature summary for 1.1.1.85 extracted from

  • Suzuki, T.; Inoki, Y.; Yamagishi, A.; Iwasaki, T.; Wakagi, T.; Oshima, T.
    Molecular and phylogenetic characterization of isopropylmalate dehydrogenase of a thermoacidophilic archaeon, Sulfolobus sp. strain 7 (1997), J. Bacteriol., 179, 1174-1179.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.85

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sulfurisphaera tokodaii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
 D-3-ethylmalate pH 8.0 Sulfurisphaera tokodaii
0.0012
-
 D-3-isopropylmalate pH 8.0 Sulfurisphaera tokodaii
0.0015
-
 D-3-isobutylmalate pH 8.0 Sulfurisphaera tokodaii
0.0023
-
 D-3-t-butylmalate pH 8.0 Sulfurisphaera tokodaii
0.0037
-
 D-3-methylmalate pH 8.0 Sulfurisphaera tokodaii
0.0058
-
 D-3-isopentylmalate pH 8.0 Sulfurisphaera tokodaii
0.39
-
 malate
-
 Sulfurisphaera tokodaii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36832
-
 4 * 37000, SDS-PAGE, 4 * 36832, calculated Sulfurisphaera tokodaii
37000
-
 4 * 37000, SDS-PAGE, 4 * 36832, calculated Sulfurisphaera tokodaii
136000
-
 sedimentation equilibrium analysis Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii P50455
-
-
Sulfurisphaera tokodaii 7 P50455
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information absence of either intra- or inter-subunit disulfide bonds Sulfurisphaera tokodaii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.9
-
 pH 8.0 Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-3-isopropylmalate + NAD+
-
 Sulfurisphaera tokodaii D-3-isopropyl-2-oxosuccinate + NADH + H+
-
 ?
(2R,3S)-3-isopropylmalate + NAD+
-
 Sulfurisphaera tokodaii 7 D-3-isopropyl-2-oxosuccinate + NADH + H+
-
 ?
D-3-ethylmalate + NAD+
-
 Sulfurisphaera tokodaii D-3-ethyl-2-oxosuccinate + NADH + H+
-
 ?
D-3-ethylmalate + NAD+
-
 Sulfurisphaera tokodaii 7 D-3-ethyl-2-oxosuccinate + NADH + H+
-
 ?
D-3-isobutylmalate + NAD+
-
 Sulfurisphaera tokodaii D-3-isobutyl-2-oxosuccinate + NADH + H+
-
 ?
D-3-isobutylmalate + NAD+
-
 Sulfurisphaera tokodaii 7 D-3-isobutyl-2-oxosuccinate + NADH + H+
-
 ?
D-3-isopentylmalate + NAD+
-
 Sulfurisphaera tokodaii D-3-isopentyl-2-oxosuccinate + NADH + H+
-
 ?
D-3-isopentylmalate + NAD+
-
 Sulfurisphaera tokodaii 7 D-3-isopentyl-2-oxosuccinate + NADH + H+
-
 ?
D-3-methylmalate + NAD+
-
 Sulfurisphaera tokodaii D-3-methyl-2-oxosuccinate + NADH + H+
-
 ?
D-3-t-butylmalate + NAD+
-
 Sulfurisphaera tokodaii D-3-t-butyl-2-oxosuccinate + NADH + H+
-
 ?
malate + NAD+
-
 Sulfurisphaera tokodaii 3-oxosuccinate + NADH + H+
-
 ?
additional information no substrate: isocitrate Sulfurisphaera tokodaii ?
-
 ?
additional information no substrate: isocitrate Sulfurisphaera tokodaii 7 ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 37000, SDS-PAGE, 4 * 36832, calculated Sulfurisphaera tokodaii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.96
-
 D-3-t-butylmalate pH 8.0 Sulfurisphaera tokodaii
2.7
-
 D-3-isopentylmalate pH 8.0 Sulfurisphaera tokodaii
3.1
-
 D-3-ethylmalate pH 8.0 Sulfurisphaera tokodaii
3.1
-
 D-3-isobutylmalate pH 8.0 Sulfurisphaera tokodaii
3.5
-
 D-3-methylmalate pH 8.0 Sulfurisphaera tokodaii
3.6
-
 D-3-isopropylmalate pH 8.0 Sulfurisphaera tokodaii
4
-
 malate
-
 Sulfurisphaera tokodaii

Cofactor

Cofactor Comment Organism Structure
NAD+ Km value 0.15 mM, NAD+ is favoured over NADP+ Sulfurisphaera tokodaii
NADP+ Km value 3.9 mM, NAD+ is favoured over NADP+ Sulfurisphaera tokodaii