Crystallization (Comment) | Organism |
---|---|
enzyme in complex with 3-isopropylmalate | Acidithiobacillus ferrooxidans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidithiobacillus ferrooxidans | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ | a large movement of domain 1 is induced by substrate binding, which results in the formation of the hydrophobic pocket for the gamma-isopropyl moiety of isopropylmalate | Acidithiobacillus ferrooxidans | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the acidic tip of IPM-Glu88 is likely to interact with the nicotinamide mononucleotide ribose of NAD+ in the ternary complex. This structure clearly explains the substrate specificity of IPMDH | Acidithiobacillus ferrooxidans |