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Literature summary for 1.1.1.85 extracted from

  • Yoda, E.; Anraku, Y.; Kirino, H.; Wakagi, T.; Oshima, T.
    Purification and characterization of 3-isopropylmalate dehydrogenase from a thermoacidophilic archaebacterium Sulfolobus sp. strain 7 (1995), FEMS Microbiol. Lett., 131, 243-247.
    View publication on PubMed

General Stability

General Stability Organism
in contrast to 3-isopropylmalate dehydrogenase from other sources, K+ and Na+ are not essential for stability of the Sulfolobus enzyme Sulfolobus sp.
K+ does not stabilize the enzyme Sulfolobus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Sulfolobus sp. 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ divalent cation required Sulfolobus sp.
Cd2+ divalent cation required, 93% of the activity with Mn2+ Sulfolobus sp.
Cd2+ required for activity, 0.2 M Sulfolobus sp.
Co2+ divalent cation required Sulfolobus sp.
Co2+ divalent cation required, 43% of the activity with Mn2+ Sulfolobus sp.
Co2+ required for activity, 0.2 M Sulfolobus sp.
K+ 1.5fold stimulation, optimal concentration: 0.2 M Sulfolobus sp.
K+ slight stimulation, but not essential. Optimal concentration 0.2 M Sulfolobus sp.
K+ about 1.5fold stimulation, optimal concentration around 0.2 M Sulfolobus sp.
Mg2+ divalent cation required Sulfolobus sp.
Mg2+ divalent cation required, 76% of the activity with Mn2+ Sulfolobus sp.
Mg2+ required for activity, 0.2 M Sulfolobus sp.
Mn2+ divalent cation required Sulfolobus sp.
Mn2+ divalent cation required, maximum activity in presence of Mn2+ Sulfolobus sp.
Mn2+ required for activity, 0.2 M Sulfolobus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
3 * 36000, or 4 * 360000, SDS-PAGE Sulfolobus sp.
36000
-
3 * or 4 * 36000, SDS-PAGE Sulfolobus sp.
110000
-
gel filtration Sulfolobus sp.
360000
-
3 * 36000, or 4 * 360000, SDS-PAGE Sulfolobus sp.

Organism

Organism UniProt Comment Textmining
Sulfolobus sp.
-
-
-
Sulfolobus sp. 7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sulfolobus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19.4
-
pH 7.0, 55°C Sulfolobus sp.
19.4
-
55°C Sulfolobus sp.

Subunits

Subunits Comment Organism
? 3 * or 4 * 36000, SDS-PAGE Sulfolobus sp.
dimer 3 * 36000, or 4 * 360000, SDS-PAGE Sulfolobus sp.
tetramer or trimer, 4* 36000, SDS-PAGE Sulfolobus sp.
trimer 3 * 36000, or 4 * 360000, SDS-PAGE Sulfolobus sp.
trimer or tetramer, 3* 36000, SDS-PAGE Sulfolobus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
assay at Sulfolobus sp.
75
-
-
Sulfolobus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
97
-
melting temperature Sulfolobus sp.
97
-
Tm-value Sulfolobus sp.
97
-
pH 7.0, 50% inactivation Sulfolobus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Sulfolobus sp.
7.5 8.5
-
Sulfolobus sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Sulfolobus sp.
NADH
-
Sulfolobus sp.

pI Value

Organism Comment pI Value Maximum pI Value
Sulfolobus sp. isoelectric focusing
-
6.9