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Literature summary for 1.1.1.85 extracted from

  • Hsu, Y.P.; Kohlhaw, G.B.
    Leucine biosynthesis in Saccharomyces cerevisiae. Purification and characterization of beta-isopropylmalate dehydrogenase (1980), J. Biol. Chem., 255, 7255-7260.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Saccharomyces cerevisiae
Ni2+
-
Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information the Km-value for NAD+ ranges from 0.054 mM to 0.15 mM when enzyme concentration varies from 0.008 mg/ml to 0.16 mg/ml, the Km-value for 3-isopropylmalate ranges from 0.023 mM to 0.042 mM when enzyme concentration varies from 0.008 mg/ml to 0.16 mg/ml Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 0.5 mM, very minor stimulatory effect Saccharomyces cerevisiae
Cd2+ divalent cation required, Mn2+ and Cd2+ are about equally effective at 0.5 mM Saccharomyces cerevisiae
Co2+ divalent cations required 0.5 mM, Co2+ is 73% as effective as Mn2+ or Co2+ Saccharomyces cerevisiae
Fe2+ 0.5 mM, very minor stimulatory effect Saccharomyces cerevisiae
Mg2+ divalent cations required 0.5 mM Mg2+ is 62% as effective as Mn2+ or Co2+ Saccharomyces cerevisiae
Mn2+ divalent cation required, Mn2+ and Cd2+ are about equally effective at 0.5 mM Saccharomyces cerevisiae
Zn2+ 0.5 mM, very minor stimulatory effect Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae SK103
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19.3
-
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-3-isopropylmalate + NAD+
-
Saccharomyces cerevisiae 2-oxoisocaproate + NADH + H+ + CO2
-
?
(2R,3S)-3-isopropylmalate + NAD+
-
Saccharomyces cerevisiae SK103 2-oxoisocaproate + NADH + H+ + CO2
-
?
additional information no activity with threo-Ds-isocitrate Saccharomyces cerevisiae ?
-
?
additional information no activity with threo-Ds-isocitrate Saccharomyces cerevisiae SK103 ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 43000-45000, enzyme exists in a dynamic monomer-dimer equilibrium, SDS-PAGE Saccharomyces cerevisiae
monomer 1 * 43000-45000, enzyme exists in a dynamic monomer-dimer equilibrium, SDS-PAGE Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
-
pH 8.3, half-life: 5 min Saccharomyces cerevisiae
23
-
pH 8.3, half-life: 5 min, essentially full protection against inactivation is provided by either 20% v/v glycerol or by simultaneous presence of Mn2+ and dithiothreitol, Mn2+ and beta-isopropylmalate or Mn2+ and alpha-ketoisocaproate Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Saccharomyces cerevisiae
9.5
-
-
Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
7.5 11.5 about 75% of maximal activity at pH 7.5 and 11.5 Saccharomyces cerevisiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.3
-
5 min, half-life at 0°C and at 23°C is 5 min Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Saccharomyces cerevisiae