Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Saccharomyces cerevisiae | |
Ni2+ | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the Km-value for NAD+ ranges from 0.054 mM to 0.15 mM when enzyme concentration varies from 0.008 mg/ml to 0.16 mg/ml, the Km-value for 3-isopropylmalate ranges from 0.023 mM to 0.042 mM when enzyme concentration varies from 0.008 mg/ml to 0.16 mg/ml | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 0.5 mM, very minor stimulatory effect | Saccharomyces cerevisiae | |
Cd2+ | divalent cation required, Mn2+ and Cd2+ are about equally effective at 0.5 mM | Saccharomyces cerevisiae | |
Co2+ | divalent cations required 0.5 mM, Co2+ is 73% as effective as Mn2+ or Co2+ | Saccharomyces cerevisiae | |
Fe2+ | 0.5 mM, very minor stimulatory effect | Saccharomyces cerevisiae | |
Mg2+ | divalent cations required 0.5 mM Mg2+ is 62% as effective as Mn2+ or Co2+ | Saccharomyces cerevisiae | |
Mn2+ | divalent cation required, Mn2+ and Cd2+ are about equally effective at 0.5 mM | Saccharomyces cerevisiae | |
Zn2+ | 0.5 mM, very minor stimulatory effect | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae SK103 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
19.3 | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ | - |
Saccharomyces cerevisiae | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? | |
(2R,3S)-3-isopropylmalate + NAD+ | - |
Saccharomyces cerevisiae SK103 | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? | |
additional information | no activity with threo-Ds-isocitrate | Saccharomyces cerevisiae | ? | - |
? | |
additional information | no activity with threo-Ds-isocitrate | Saccharomyces cerevisiae SK103 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 43000-45000, enzyme exists in a dynamic monomer-dimer equilibrium, SDS-PAGE | Saccharomyces cerevisiae |
monomer | 1 * 43000-45000, enzyme exists in a dynamic monomer-dimer equilibrium, SDS-PAGE | Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
pH 8.3, half-life: 5 min | Saccharomyces cerevisiae |
23 | - |
pH 8.3, half-life: 5 min, essentially full protection against inactivation is provided by either 20% v/v glycerol or by simultaneous presence of Mn2+ and dithiothreitol, Mn2+ and beta-isopropylmalate or Mn2+ and alpha-ketoisocaproate | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Saccharomyces cerevisiae |
9.5 | - |
- |
Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 11.5 | about 75% of maximal activity at pH 7.5 and 11.5 | Saccharomyces cerevisiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
5 min, half-life at 0°C and at 23°C is 5 min | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Saccharomyces cerevisiae |