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Literature summary for 1.1.1.83 extracted from

  • Lähdesmäki, M.; Mäntsälä, P.
    Comparison of D-malate and beta, beta-dimethylmalate dehydrogenases from Pseudomonas fluorescens UK-1 (1980), Biochim. Biophys. Acta, 613, 266-274.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,2-Butanedione
-
Pseudomonas fluorescens
N-ethylmaleimide 1 mM, 18% inactivation Pseudomonas fluorescens
p-chloromercuribenzoate 0.2 mM, 10% inactivation Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.49
-
NAD+
-
Pseudomonas fluorescens
2.2
-
D-malate
-
Pseudomonas fluorescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
4 * 34000, SDS-PAGE Pseudomonas fluorescens
140000
-
gel filtration Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
UK-1
-
Pseudomonas fluorescens UK-1
-
UK-1
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.02
-
-
Pseudomonas fluorescens

Storage Stability

Storage Stability Organism
-70°C, pH 7.2, 1 mM EDTA Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-malate + NAD+
-
Pseudomonas fluorescens pyruvate + CO2 + NADH
-
?
(R)-malate + NAD+
-
Pseudomonas fluorescens UK-1 pyruvate + CO2 + NADH
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 34000, SDS-PAGE Pseudomonas fluorescens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
NAD+ provides better protection against inactivation than D-malate or beta,beta-dimethyl-DL-malate Pseudomonas fluorescens
65
-
2 min, 64% loss of activity Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
-
Pseudomonas fluorescens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas fluorescens