Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,2-Butanedione | - |
Pseudomonas fluorescens | |
N-ethylmaleimide | 1 mM, 18% inactivation | Pseudomonas fluorescens | |
p-chloromercuribenzoate | 0.2 mM, 10% inactivation | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.49 | - |
NAD+ | - |
Pseudomonas fluorescens | |
2.2 | - |
D-malate | - |
Pseudomonas fluorescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
4 * 34000, SDS-PAGE | Pseudomonas fluorescens |
140000 | - |
gel filtration | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
UK-1 | - |
Pseudomonas fluorescens UK-1 | - |
UK-1 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas fluorescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.02 | - |
- |
Pseudomonas fluorescens |
Storage Stability | Organism |
---|---|
-70°C, pH 7.2, 1 mM EDTA | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-malate + NAD+ | - |
Pseudomonas fluorescens | pyruvate + CO2 + NADH | - |
? | |
(R)-malate + NAD+ | - |
Pseudomonas fluorescens UK-1 | pyruvate + CO2 + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 34000, SDS-PAGE | Pseudomonas fluorescens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
NAD+ provides better protection against inactivation than D-malate or beta,beta-dimethyl-DL-malate | Pseudomonas fluorescens |
65 | - |
2 min, 64% loss of activity | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
- |
Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas fluorescens |