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Literature summary for 1.1.1.82 extracted from

  • Fickenscher, K.; Scheibe, R.
    Limited proteolysis of inactive tetrameric chloroplast NADP-malate dehydrogenase produces active dimers (1988), Arch. Biochem. Biophys., 260, 771-779.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
acetone increases the activity of the oxidized truncated enzyme form, optimal concentration 15% Pisum sativum
carboxypeptidase Y incubation of the native oxidized enzyme at pH 6.0, 25°C, results in slow activation. A small carboxy-terminal peptide of the native enzyme is accessible to proteolytic degradation followed by activation of the inactive oxidized enzyme. This peptide is involved in the regulation of activity, tetramer formation and thioredoxin binding Pisum sativum
dithiothreitol activates Pisum sativum
methanol increases the activity of the oxidized truncated enzyme form, optimal concentration 8% Pisum sativum

General Stability

General Stability Organism
a small carboxy-terminal peptide of the native enzyme is accessible to proteolytic degradation followed by activation of the inactive oxidized enzyme. This peptide is involved in the regulation of activity, tetramer formation and thioredoxin binding Pisum sativum
denaturation by 2 M guanidine-HCl Pisum sativum

Inhibitors

Inhibitors Comment Organism Structure
KCl oxidized truncated enzyme Pisum sativum
oxaloacetate pH 8, substrate inhibition of the reduced truncated enzyme Pisum sativum
Urea oxidized truncated enzyme Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.039
-
NADPH reduced native enzyme Pisum sativum
0.042
-
oxaloacetate reduced truncated enzyme Pisum sativum
0.046
-
NADPH oxidized truncated enzyme Pisum sativum
0.048
-
oxaloacetate reduced native enzyme Pisum sativum
0.089
-
NADPH reduced truncated enzyme Pisum sativum
1.2
-
oxaloacetate oxidized truncated enzyme Pisum sativum

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Pisum sativum 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38900
-
4 * 38900, native enzyme, SDS-PAGE Pisum sativum

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification a small carboxy-terminal peptide of the native enzyme is accessible to proteolytic degradation followed by activation of the inactive oxidized enzyme. This peptide is involved in the regulation of activity, tetramer formation and thioredoxin binding Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxaloacetate + NADPH
-
Pisum sativum (S)-malate + NADP+
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 38900, native enzyme, SDS-PAGE Pisum sativum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5 reduction of oxaloacetate, oxidized truncated enzyme Pisum sativum
8 8.5 active enzyme Pisum sativum