Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by 2 mM acetohydroxamate | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
Hydroxypyruvate | at pH 6.0 | Spinacia oleracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
2 * 38000, SDS-PAGE | Spinacia oleracea |
70000 | - |
gel filtration | Spinacia oleracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxypyruvate + NAD(P)H | Spinacia oleracea | - |
D-glycerate + NAD(P)+ + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
257 | - |
- |
Spinacia oleracea |
Storage Stability | Organism |
---|---|
-20°C, 20 mM MOPS, pH 7.1, 14 mM 2-mercaptoethanol, 50% glycerol | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + NAD(P)H | activity with hydroxypyruvate and NADPH is 2fold higher than with other pair of reactants | Spinacia oleracea | glycolate + NAD(P)+ | - |
ir | |
hydroxypyruvate + NAD(P)H | - |
Spinacia oleracea | D-glycerate + NAD(P)+ + H+ | - |
? | |
hydroxypyruvate + NAD(P)H | - |
Spinacia oleracea | D-glycerate + NAD(P)+ + H+ | - |
ir |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38000, SDS-PAGE | Spinacia oleracea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | strong decrease above | Spinacia oleracea |