Inhibitors | Comment | Organism | Structure |
---|---|---|---|
citrate | 5 mM, 20% inhibition of NADH linked hydroxypyruvate reduction, 25% of NADPH linked reduction | Methylorubrum extorquens AM1 | |
D-glycerate | 5 mM, 20% inhibition of NADH linked hydroxypyruvate reduction, 40% of NADPH linked reduction | Methylorubrum extorquens AM1 | |
glycolate | 5 mM, 15% of NADPH linked reduction | Methylorubrum extorquens AM1 | |
glyoxylate | 10 mM, 55% inhibition of NADH linked hydroxypyruvate reduction, 15% of NADPH linked reduction, 80% of NAD+ linked glycerate oxidation | Methylorubrum extorquens AM1 | |
Hydroxypyruvate | 0.5 mM, 90% of NAD+ linked glycerate oxidation | Methylorubrum extorquens AM1 | |
NAD+ | 4 mM, 10% inhibition of NADH linked hydroxypyruvate reduction, 60% of NADPH linked reduction | Methylorubrum extorquens AM1 | |
NADP+ | 4 mM, 15% inhibition of NADH linked hydroxypyruvate reduction, 40% of NADPH linked reduction, 8% of NAD+ linked glycerate oxidation | Methylorubrum extorquens AM1 | |
oxaloacetate | 2.5 mM, 10% inhibition of NADH linked hydroxypyruvate reduction, 20% of NADPH linked reduction | Methylorubrum extorquens AM1 | |
pyruvate | 10 mM, 40% inhibition of NADH linked hydroxypyruvate reduction | Methylorubrum extorquens AM1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
NADH | - |
Methylorubrum extorquens AM1 | |
0.06 | - |
NADPH | - |
Methylorubrum extorquens AM1 | |
0.1 | - |
Hydroxypyruvate | cofactor NADH | Methylorubrum extorquens AM1 | |
1.25 | - |
Hydroxypyruvate | cofactor NADPH | Methylorubrum extorquens AM1 | |
2.6 | - |
glycerate | - |
Methylorubrum extorquens AM1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
2 * 37000, SDS-PAGE | Methylorubrum extorquens AM1 |
71000 | - |
gradient PAGE | Methylorubrum extorquens AM1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxypyruvate + NAD(P)H | Methylorubrum extorquens AM1 | cofactor NADPH, 80% of activity with NADH, rate of oxidation reaction: 1.5% of reduction reaction only with cofactor NADH | D-glycerate + NAD(P)+ + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylorubrum extorquens AM1 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
675 | - |
- |
Methylorubrum extorquens AM1 |
Storage Stability | Organism |
---|---|
4°C, 50 mM Tris-HCl, pH 7.5, 10% glycerol, 1 mM dithiothreitol, 2 months, 10% loss of activity | Methylorubrum extorquens AM1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glycerate + NAD+ | - |
Methylorubrum extorquens AM1 | 3-hydroxypyruvate + NADH + H+ | - |
r | |
glyoxylate + NAD(P)H | cofactor NADH, 15% of activity with hydroxypyruvate, cofactor NADPH, 1.5% of activity with hydroxypyruvate | Methylorubrum extorquens AM1 | glycolate + NAD(P)+ | - |
? | |
hydroxypyruvate + NAD(P)H | - |
Methylorubrum extorquens AM1 | D-glycerate + NAD(P)+ + H+ | - |
? | |
hydroxypyruvate + NAD(P)H | cofactor NADPH, 80% of activity with NADH, rate of oxidation reaction: 1.5% of reduction reaction only with cofactor NADH | Methylorubrum extorquens AM1 | D-glycerate + NAD(P)+ + H+ | - |
r | |
hydroxypyruvate + NADH + H+ | - |
Methylorubrum extorquens AM1 | D-glycerate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37000, SDS-PAGE | Methylorubrum extorquens AM1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
broad optimum between pH 4.0 and 6.5 for hydroxypyruvate reduction | Methylorubrum extorquens AM1 |
9 | 11 | glycerate oxidation | Methylorubrum extorquens AM1 |