Cloned (Comment) | Organism |
---|---|
gene YDL022W, phylogenetic analysis, expression in Escherichia coli strain BL21 | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
purified recombinant untagged enzyme, hanging drop vapour diffusion method using a reservoir solution consisting of 12% PEG 8000, 0.1 M Tris-HCl, pH 8.5, 0.3 M MgCl2, X-ray diffraction structure determination and analysis at 2.45 A resolution, molecular replacement and modeling | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycerol 3-phosphate + NAD+ | Saccharomyces cerevisiae | - |
glycerone phosphate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q00055 | gene YDL022W encoding GPD1 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21 by anion exchange chromatography to over 95% purity | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycerol 3-phosphate + NAD+ | - |
Saccharomyces cerevisiae | glycerone phosphate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme monomer is organized with N- and C-terminal domains. The N-terminal domain contains a classic Rossmann fold with the (beta-alpha-beta-alpha-beta)2 motif typical of many NAD+-dependent enzymes, while the C-terminal domain is mainly alpha-helical, structure comparisons, overview | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
glycerol-3-phosphate dehydrogenase | - |
Saccharomyces cerevisiae |
GPD1 | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent on | Saccharomyces cerevisiae | |
NADH | dependent on | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
evolution | structural and phylogenetic comparisons reveal four main structure types among the five families of glycerol-3-phosphate and glycerol-1-phosphate dehydrogenases, overview | Saccharomyces cerevisiae |
metabolism | the interconversion of glycerol 3-phosphate and dihydroxyacetone phosphate by glycerol-3-phosphate dehydrogenases provides a link between carbohydrate and lipid metabolism. Glycerol 3-phosphate from the breakdown of phospholipids and triglycerides (via glycerol kinase) is converted into the glycolysis intermediate dihydroxyacetone phosphate, while the reverse reaction produces glycerol 3-phosphate, which is required for the synthesis of triglycerides and phospholipids | Saccharomyces cerevisiae |
physiological function | the interconversion of glycerol 3-phosphate and dihydroxyacetone phosphate by glycerol-3-phosphate dehydrogenases provides Saccharomyces cerevisiae with protection against osmotic and anoxic stress. The concerted action of cytosolic (NAD+-dependent) G3PDHs and membrane-bound (FAD-dependent) G3PDHs transfers reducing equivalents from cytosolic NADH into the electron-transport chain of both bacteria and mitochondria | Saccharomyces cerevisiae |