Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cl- | cations, i.e. H+, K+, Na+ associated with Cl- do not affect the reduction of dihydroxyacetone phosphate | Saccharomyces cerevisiae | |
additional information | - |
Saccharomyces cerevisiae | |
NaCl | inactivates irreversibly | Saccharomyces cerevisiae | |
phosphate | - |
Saccharomyces cerevisiae | |
TEA-buffer | - |
Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
x * 31000, gel filtration, estimated from elution volume on sepharose 6B | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycerol 3-phosphate + NAD+ | Saccharomyces cerevisiae | reverse reaction favoured direction | dihydroxyacetone phosphate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
158 | - |
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20-0°C, stable in 75% ammonium sulfate | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycerol 3-phosphate + NAD+ | - |
Saccharomyces cerevisiae | dihydroxyacetone phosphate + NADH + H+ | - |
r | |
sn-glycerol 3-phosphate + NAD+ | reverse reaction favoured direction | Saccharomyces cerevisiae | dihydroxyacetone phosphate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, gel filtration, estimated from elution volume on sepharose 6B | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7.2 | activity falls off rapidly as pH is increased | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADPH | Saccharomyces cerevisiae | |
NAD+ | - |
Saccharomyces cerevisiae | |
NADH | - |
Saccharomyces cerevisiae |