Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-chloromercuribenzoate | 10 nM, strong inhibition | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | various Km-values of 4 isozymes of heart, muscle, liver and mammary gland | Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Oryctolagus cuniculus | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37500 | - |
x * 37500, isozyme from heart (II6.1), calculated by amino acid composition | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycerol 3-phosphate + NAD+ | Oryctolagus cuniculus | favoured reaction of heart isozyme II6.1 | dihydroxyacetone phosphate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
several isozymes, distinct in isoelectric points | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Brown Pearce carcinoma cell | - |
Oryctolagus cuniculus | - |
liver | - |
Oryctolagus cuniculus | - |
mammary gland | - |
Oryctolagus cuniculus | - |
muscle | heart muscle | Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
115 | - |
heart | Oryctolagus cuniculus |
180 | - |
mammary gland | Oryctolagus cuniculus |
Storage Stability | Organism |
---|---|
4°C, 20% v/v, glycerol, purified stable | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycerol 3-phosphate + NAD+ | - |
Oryctolagus cuniculus | dihydroxyacetone phosphate + NADH + H+ | - |
r | |
sn-glycerol 3-phosphate + NAD+ | favoured reaction of heart isozyme II6.1 | Oryctolagus cuniculus | dihydroxyacetone phosphate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 37500, isozyme from heart (II6.1), calculated by amino acid composition | Oryctolagus cuniculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
48 | - |
t1/2: 2 min, heart isozyme II6.1 | Oryctolagus cuniculus |
48 | - |
t1/2: 2 min, brain isozyme I5.9 | Oryctolagus cuniculus |
48 | - |
t1/2: 15 min, muscle isozyme I6.5 | Oryctolagus cuniculus |
50 | - |
t1/2: 2 min in heart and 10 min in muscle, mammary gland and liver | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADPH | Oryctolagus cuniculus | |
additional information | not NADP+ | Oryctolagus cuniculus | |
NAD+ | - |
Oryctolagus cuniculus | |
NADH | - |
Oryctolagus cuniculus |