Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli JM109, stereospecificity of resulting L-butanediol dehydrogenase is reduced. Expressed gene contains tac-promoter and upstream region of the meso-butanediol dehydrogenase gene of Klebsiella pneumoniae IAM1063. A more stable stereospecificity is exhibited | Corynebacterium glutamicum |
When the enzyme gene is only expressed in Escherichia coli using the tac promoter, the stereospecificity of resulting enzyme gene is reduced. When the region upstream of the meso-butanediol dehydrogenase gene of Klebsiella pneumoniae is also involved in the expression of gene, the resulting enzyme exhibits more stable stereospecificity. | Corynebacterium glutamicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | - |
C-1012 | - |
Corynebacterium glutamicum C-1012 | - |
C-1012 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
highest specific activities without glucose addition to culture medium, induction with 0.25 mg/ml isopropyl-beta-thiogalactopyranosid | Corynebacterium glutamicum |
1.51 | - |
plasmid containing L-butanediol dehydrogenase gene | Corynebacterium glutamicum |
17 | - |
plasmid containing tac promoter and L-butanediol dehydrogenase ORF | Corynebacterium glutamicum |
18.6 | - |
plasmid containing meso-butanediol dehydrogenase promoter region and L-butanediol dehydrogenase ORF | Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-acetoin + NADH | short chain dehydrogenase reductase family | Corynebacterium glutamicum | L-2,3-butanediol | - |
r | |
L-acetoin + NADH | exhibits marked sequence similarity and common functionally conserved sequence with meso-enzyme | Corynebacterium glutamicum | L-2,3-butanediol | - |
r | |
L-acetoin + NADH | short chain dehydrogenase reductase family | Corynebacterium glutamicum C-1012 | L-2,3-butanediol | - |
r | |
L-acetoin + NADH | exhibits marked sequence similarity and common functionally conserved sequence with meso-enzyme | Corynebacterium glutamicum C-1012 | L-2,3-butanediol | - |
r | |
L-acetoin + NADH + H+ | To confirm the high production of enzyme, the conversion of L-acetoin, in a racemic mixture, to L-2,3-butanediol is studied. 0.37% L-2,3-butanediol is formed from 1% L-acetoin added to the culture. | Corynebacterium glutamicum | (S,S)-butanediol + NAD+ | - |
? | |
L-acetoin + NADH + H+ | To confirm the high production of enzyme, the conversion of L-acetoin, in a racemic mixture, to L-2,3-butanediol is studied. 0.37% L-2,3-butanediol is formed from 1% L-acetoin added to the culture. | Corynebacterium glutamicum C-1012 | (S,S)-butanediol + NAD+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Corynebacterium glutamicum | |
NADH | - |
Corynebacterium glutamicum |