Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.75 extracted from

  • Pickard, M.A.; Higgins, I.J.; Turner, J.M.
    Purification and properties of l-1-aminopropan-2-ol. NAD oxidoreductase from a pseudomonad grown on DL-1-aminopropan-2-ol (1968), J. Gen. Microbiol., 54, 115-126.
    View publication on PubMed

General Stability

General Stability Organism
dialysis overnight at 4°C, no loss of activity Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
(R)-1-aminopropan-2-ol competitive Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
D,L-1-aminopropan-2-ol
-
Pseudomonas sp.
0.2
-
NADP+ with D,L-1-aminopropan-2-ol as substrate Pseudomonas sp.
0.3
-
NAD+ with D,L-1-aminopropan-2-ol as substrate Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000 80000 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-1-aminopropan-2-ol + NAD+ Pseudomonas sp. involved in L-threonine and vitamin B12 pathway aminoacetone + NADH
-
r

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.001
-
with 1-aminopropanol as growth substrate Pseudomonas sp.
0.4
-
crude extract Pseudomonas sp.
8.5
-
purified enzyme Pseudomonas sp.

Storage Stability

Storage Stability Organism
-15°C phosphate buffer, pH 7.2, 1 mg protein/ml, stable for more than 1 year Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-diaminopropan-2-ol + NAD+ poor substrate Pseudomonas sp. 2-aminoacetamide + NADH
-
?
D,L-1-aminopropan-2-ol + NAD+
-
Pseudomonas sp. aminoacetone + NADH
-
?
D,L-2-hydroxy-2-phenylethylamine + NAD+
-
Pseudomonas sp. 2-amino-1-phenylethanone + NADH
-
?
D,L-3-hydroxybutyrate + NAD+
-
Pseudomonas sp. ?
-
?
D,L-aminobutan-2-ol + NAD+
-
Pseudomonas sp. 1-amino-2-oxobutane + NADH
-
?
D,L-phenylserine + NAD+
-
Pseudomonas sp. ?
-
?
L-1-aminopropan-2-ol + NAD+ no activity with D-enantiomer Pseudomonas sp. aminoacetone + NADH
-
r
L-1-aminopropan-2-ol + NAD+ involved in L-threonine and vitamin B12 pathway Pseudomonas sp. aminoacetone + NADH
-
r
L-1-aminopropan-2-ol + NADP+ about the same activity as with NAD+ as cofactor Pseudomonas sp. aminoacetone + NADPH
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 10.5 low activity at pH 6, 80% of activity at pH 10.5 Pseudomonas sp.