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Literature summary for 1.1.1.72 extracted from

  • Richter, N.; Breicha, K.; Hummel, W.; Niefind, K.
    The Three-Dimensional Structure of AKR11B4, a Glycerol Dehydrogenase from Gluconobacter oxydans, Reveals a Tryptophan Residue as an Accelerator of Reaction Turnover (2010), J. Mol. Biol., 404, 353-362.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged version Gluconobacter oxydans

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop variant of the vapor diffusion method Gluconobacter oxydans

Protein Variants

Protein Variants Comment Organism
W23A W23 is a key residue (accelerator) for NADP/NADPH turnover Gluconobacter oxydans
W23M W23 is a key residue (accelerator) for NADP/NADPH turnover Gluconobacter oxydans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information KM-value 118.0 mM with substrate DL-glyceraldehyde, W23A mutant protein, pH 7.0, 30°C Gluconobacter oxydans
additional information
-
additional information KM-value 2.4 mM with substrate DL-glyceraldehyde, wild-type protein, pH 7.0, 30°C Gluconobacter oxydans
additional information
-
additional information KM-value 49.4 mM with substrate DL-glyceraldehyde, W23M mutant protein, pH 7.0, 30°C Gluconobacter oxydans
0.0041
-
NADPH wild-type protein, pH 7.0, 30°C Gluconobacter oxydans
0.0062
-
NADPH W23A mutant protein, pH 7.0, 30°C Gluconobacter oxydans
0.0062
-
NADPH W23M mutant protein, pH 7.0, 30°C Gluconobacter oxydans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37213
-
37213, calculated Gluconobacter oxydans

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans Q5FQJ0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Gluconobacter oxydans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2
-
W23A mutant protein, NADPH as variable substrate, pH 7.0, 30°C Gluconobacter oxydans
4.9
-
W23A mutant protein, DL-glyceraldehyde as variable substrate, pH 7.0, 30°C Gluconobacter oxydans
37
-
W23M mutant protein, DL-glyceraldehyde as variable substrate, pH 7.0, 30°C Gluconobacter oxydans
54
-
W23M mutant protein, NADPH as variable substrate, pH 7.0, 30°C Gluconobacter oxydans
80.5
-
wild-type protein, DL-glyceraldehyde as variable substrate, pH 7.0, 30°C Gluconobacter oxydans
87.1
-
wild-type protein, NADPH as variable substrate, pH 7.0, 30°C Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-glyceraldehyde + NADPH + H+ significant enantioselectivity Gluconobacter oxydans glycerol + NADP+
-
r
glycerol + NADP+
-
Gluconobacter oxydans D-glyceraldehyde + NADPH + H+ significant enantioselectivity r

Subunits

Subunits Comment Organism
monomer 37213, calculated Gluconobacter oxydans

Synonyms

Synonyms Comment Organism
AKR11B4
-
Gluconobacter oxydans
glycerol dehydrogenase
-
Gluconobacter oxydans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information 24.2/sec with substrate D/L-glyceraldehyde, W23M mutant protein, pH 7.0, 30°C Gluconobacter oxydans
additional information
-
additional information 3.2/sec with substrate D/L-glyceraldehyde, W23A mutant protein, pH 7.0, 30°C Gluconobacter oxydans
additional information
-
additional information 52.8/sec with substrate D/L-glyceraldehyde, wild-type protein, pH 7.0, 30°C Gluconobacter oxydans
2.7
-
NADPH W23A mutant protein, pH 7.0, 30°C Gluconobacter oxydans
35.4
-
NADPH W23M mutant protein, pH 7.0, 30°C Gluconobacter oxydans
57.2
-
NADPH wild-type protein, pH 7.0, 30°C Gluconobacter oxydans