Cloned (Comment) | Organism |
---|---|
His-tagged version | Gluconobacter oxydans |
Crystallization (Comment) | Organism |
---|---|
sitting-drop variant of the vapor diffusion method | Gluconobacter oxydans |
Protein Variants | Comment | Organism |
---|---|---|
W23A | W23 is a key residue (accelerator) for NADP/NADPH turnover | Gluconobacter oxydans |
W23M | W23 is a key residue (accelerator) for NADP/NADPH turnover | Gluconobacter oxydans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | KM-value 118.0 mM with substrate DL-glyceraldehyde, W23A mutant protein, pH 7.0, 30°C | Gluconobacter oxydans | |
additional information | - |
additional information | KM-value 2.4 mM with substrate DL-glyceraldehyde, wild-type protein, pH 7.0, 30°C | Gluconobacter oxydans | |
additional information | - |
additional information | KM-value 49.4 mM with substrate DL-glyceraldehyde, W23M mutant protein, pH 7.0, 30°C | Gluconobacter oxydans | |
0.0041 | - |
NADPH | wild-type protein, pH 7.0, 30°C | Gluconobacter oxydans | |
0.0062 | - |
NADPH | W23A mutant protein, pH 7.0, 30°C | Gluconobacter oxydans | |
0.0062 | - |
NADPH | W23M mutant protein, pH 7.0, 30°C | Gluconobacter oxydans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37213 | - |
37213, calculated | Gluconobacter oxydans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | Q5FQJ0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Gluconobacter oxydans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.2 | - |
W23A mutant protein, NADPH as variable substrate, pH 7.0, 30°C | Gluconobacter oxydans |
4.9 | - |
W23A mutant protein, DL-glyceraldehyde as variable substrate, pH 7.0, 30°C | Gluconobacter oxydans |
37 | - |
W23M mutant protein, DL-glyceraldehyde as variable substrate, pH 7.0, 30°C | Gluconobacter oxydans |
54 | - |
W23M mutant protein, NADPH as variable substrate, pH 7.0, 30°C | Gluconobacter oxydans |
80.5 | - |
wild-type protein, DL-glyceraldehyde as variable substrate, pH 7.0, 30°C | Gluconobacter oxydans |
87.1 | - |
wild-type protein, NADPH as variable substrate, pH 7.0, 30°C | Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-glyceraldehyde + NADPH + H+ | significant enantioselectivity | Gluconobacter oxydans | glycerol + NADP+ | - |
r | |
glycerol + NADP+ | - |
Gluconobacter oxydans | D-glyceraldehyde + NADPH + H+ | significant enantioselectivity | r |
Subunits | Comment | Organism |
---|---|---|
monomer | 37213, calculated | Gluconobacter oxydans |
Synonyms | Comment | Organism |
---|---|---|
AKR11B4 | - |
Gluconobacter oxydans |
glycerol dehydrogenase | - |
Gluconobacter oxydans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 24.2/sec with substrate D/L-glyceraldehyde, W23M mutant protein, pH 7.0, 30°C | Gluconobacter oxydans | |
additional information | - |
additional information | 3.2/sec with substrate D/L-glyceraldehyde, W23A mutant protein, pH 7.0, 30°C | Gluconobacter oxydans | |
additional information | - |
additional information | 52.8/sec with substrate D/L-glyceraldehyde, wild-type protein, pH 7.0, 30°C | Gluconobacter oxydans | |
2.7 | - |
NADPH | W23A mutant protein, pH 7.0, 30°C | Gluconobacter oxydans | |
35.4 | - |
NADPH | W23M mutant protein, pH 7.0, 30°C | Gluconobacter oxydans | |
57.2 | - |
NADPH | wild-type protein, pH 7.0, 30°C | Gluconobacter oxydans |