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Literature summary for 1.1.1.69 extracted from
- Investigation of 5-ketogluconate formation by Gluconobacter oxydans (1992), DECHEMA Biotechnol. Conf., 5, 109-112.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli and Gluconobacter oxydans | Gluconobacter oxydans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 1 mM, 40% loss of activity | Gluconobacter oxydans |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.073 | - |
NADP+ | - |
Gluconobacter oxydans | |
| 20.6 | - |
D-gluconate | - |
Gluconobacter oxydans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Gluconobacter oxydans | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 1 mM, increases the activity to 127% | Gluconobacter oxydans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
gel filtration | Gluconobacter oxydans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gluconobacter oxydans | - |
subsp. oxydans DSM3503 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Gluconobacter oxydans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Gluconobacter oxydans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-dehydro-D-gluconate + NADPH | - |
Gluconobacter oxydans | D-gluconate + NADP+ | - |
r | |
| D-fructose + NADPH | 10% of the activity with 5-dehydrydro-D-gluconate | Gluconobacter oxydans | 5-dehydro-D-fructose + NADP+ | - |
? | |
| D-gluconate + NADP+ | - |
Gluconobacter oxydans | 5-dehydro-D-gluconate + NADPH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 75000, SDS-PAGE | Gluconobacter oxydans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
reduction of 5-dehydro-D-gluconate | Gluconobacter oxydans |
| 10 | - |
- |
Gluconobacter oxydans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | cofactor | Gluconobacter oxydans | |
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