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Literature summary for 1.1.1.69 extracted from
- 5-Ketogluconic acid reductase (1966), Methods Enzymol., 9, 200-203.
No PubMed abstract available
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-dehydro-D-gluconate + NADPH | Gluconobacter oxydans | - |
D-gluconate + NADP+ | - |
? |  |
| 5-dehydro-D-gluconate + NADPH | Escherichia coli | inducible enzyme | D-gluconate + NADP+ | - |
? | |
| 5-dehydro-D-gluconate + NADPH | Klebsiella sp. | inducible enzyme | D-gluconate + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Gluconobacter oxydans | - |
- |
- |
| Klebsiella sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Gluconobacter oxydans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-dehydro-D-gluconate + NADH | reaction proceeds twice as fast with NADH as with NADPH | Escherichia coli | D-gluconate + NAD+ | - |
? | |
| 5-dehydro-D-gluconate + NADH | reaction proceeds twice as fast with NADH as with NADPH | Klebsiella sp. | D-gluconate + NAD+ | - |
? | |
| 5-dehydro-D-gluconate + NADH | no reaction with NADH | Gluconobacter oxydans | D-gluconate + NAD+ | - |
? | |
| 5-dehydro-D-gluconate + NADPH | - |
Escherichia coli | D-gluconate + NADP+ | - |
? | |
| 5-dehydro-D-gluconate + NADPH | - |
Gluconobacter oxydans | D-gluconate + NADP+ | - |
? | |
| 5-dehydro-D-gluconate + NADPH | - |
Klebsiella sp. | D-gluconate + NADP+ | - |
? | |
| 5-dehydro-D-gluconate + NADPH | inducible enzyme | Escherichia coli | D-gluconate + NADP+ | - |
? | |
| 5-dehydro-D-gluconate + NADPH | inducible enzyme | Klebsiella sp. | D-gluconate + NADP+ | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | reduction of 5-dehydro-D-gluconate | Escherichia coli |
| 6 | 7 | reduction of 5-dehydro-D-gluconate | Klebsiella sp. |
| 7.3 | 7.5 | reduction of 5-dehydro-D-gluconate | Gluconobacter oxydans |
| 9.3 | - |
oxidation of D-gluconate | Gluconobacter oxydans |
| 10 | 11 | oxidation of D-gluconate | Escherichia coli |
| 10 | 11 | oxidation of D-gluconate | Klebsiella sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | cofactor | Escherichia coli | |
| NADH | cofactor | Klebsiella sp. | |
| NADPH | cofactor | Escherichia coli | |
| NADPH | cofactor | Gluconobacter oxydans | |
| NADPH | cofactor | Klebsiella sp. | |
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Release 2023.1 (January 2023)
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