Protein Variants | Comment | Organism |
---|---|---|
K295A | 30000fold lower turnover number for D-mannitol oxidation at pH 10.0 than the wild-type enzyme | Pseudomonas fluorescens |
K295M | 2000000fold lower turnover number for D-mannitol oxidation at pH 10.0 than the wild-type enzyme | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol + NAD+ | the epsilon-NH2 group of Lys295 participates in an obligatory pH-dependent, pre-catalytic equilibrium which may control alcohol/alkoxide equilibration of the enzyme-bound D-mannitol and activates the C2 atom for subsequent catalytic oxidation by NAD+ | Pseudomonas fluorescens | D-fructose + NADH + H+ | - |
? |