Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Schizosaccharomyces pombe |
General Stability | Organism |
---|---|
purified enzyme very unstable but can be stabilized by low concentrations of various detergents such as Tween 40 | Schizosaccharomyces pombe |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.096 | - |
2-nitrobenzaldehyde | - |
Schizosaccharomyces pombe | |
0.9 | - |
pyridoxal | - |
Schizosaccharomyces pombe |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
gel filtration | Schizosaccharomyces pombe |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyridoxine + NADP+ | Schizosaccharomyces pombe | - |
pyridoxal + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | O14295 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Schizosaccharomyces pombe |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrobenzaldehyde + NADPH | - |
Schizosaccharomyces pombe | 2-nitrobenzyl alcohol + NADP+ | - |
? | |
pyridoxine + NADP+ | - |
Schizosaccharomyces pombe | pyridoxal + NADPH | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | for formation of pyridoxine | Schizosaccharomyces pombe |
7.5 | 8.5 | for formation of pyridoxal | Schizosaccharomyces pombe |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Schizosaccharomyces pombe | |
NADPH | - |
Schizosaccharomyces pombe |