Cloned (Comment) | Organism |
---|---|
gene AKR1B15 located on chromosome 7, cloning of two isoforms: AKR1B15.1 and AKR1B15.2, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged isozymes in Escherichia coli strain BL21(DE3), recombinant expression of C- or N-terminally c-Myc-tagged isozymes in HEK-293 cells | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics, binding of dinucleotide cofactors to AKR1B15.1, overview | Homo sapiens | |
0.0019 | - |
DELTA4-3,17-androstendione | pH 7.4, 37°C recombinant His-tagged enzyme | Homo sapiens | |
0.0025 | - |
estrone | pH 7.4, 37°C recombinant His-tagged enzyme | Homo sapiens | |
0.0028 | - |
androsterone | pH 7.4, 37°C recombinant His-tagged enzyme | Homo sapiens | |
0.0071 | - |
testosterone | pH 7.4, 37°C recombinant His-tagged enzyme | Homo sapiens | |
0.0091 | - |
17beta-estradiol | pH 7.4, 37°C recombinant His-tagged enzyme | Homo sapiens | |
0.0192 | - |
5alpha-androstane 3alpha,17beta-diol | pH 7.4, 37°C recombinant His-tagged enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | isozyme AKR1B15.2 | Homo sapiens | 5829 | - |
mitochondrion | isozyme AKR1B15.1 | Homo sapiens | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
17beta-estradiol + NADP+ | Homo sapiens | - |
estrone + NADPH + H+ | - |
? | |
5alpha-androstane-3alpha,17beta-diol + NAD+ | Homo sapiens | - |
androsterone + NADH + H+ | - |
? | |
testosterone + NAD+ | Homo sapiens | - |
DELTA4-3,17-androstendione + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | C9JRZ8 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged isozymes AKR1B15.1 and AKR1B15.2 isozymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adipose tissue | isozymes AKR1B15.1 and AKR1B15.2 | Homo sapiens | - |
brain | fetal, very low expression of isozyme AKR1B15.2 | Homo sapiens | - |
heart | very low expression of isozymes AKR1B15.1 and AKR1B15.2 | Homo sapiens | - |
HEK-293 cell | isozymes AKR1B15.1 and AKR1B15.2 | Homo sapiens | - |
additional information | isozyme tissue-specific expression analysis, overview | Homo sapiens | - |
ovary | low expression of isozymes AKR1B15.1 and AKR1B15.2 | Homo sapiens | - |
placenta | isozymes AKR1B15.1 and AKR1B15.2 | Homo sapiens | - |
prostate | isozyme AKR1B15.1 | Homo sapiens | - |
skeletal muscle | isozymes AKR1B15.1 and AKR1B15.2 | Homo sapiens | - |
testis | isozyme AKR1B15.1, very low expression of isozyme AKR1B15.2 | Homo sapiens | - |
thymus | isozymes AKR1B15.1 and AKR1B15.2 | Homo sapiens | - |
thyroid gland | isozyme AKR1B15.2 | Homo sapiens | - |
uterus | very low expression of isozymes AKR1B15.1 and AKR1B15.2 | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17beta-estradiol + NADP+ | - |
Homo sapiens | estrone + NADPH + H+ | - |
? | |
5alpha-androstane-3alpha,17beta-diol + NAD+ | - |
Homo sapiens | androsterone + NADH + H+ | - |
? | |
androst-4-ene-3,17-dione + NADH + H+ | - |
Homo sapiens | testosterone + NAD+ | - |
? | |
androsterone + NADH + H+ | - |
Homo sapiens | 5alpha-androstane-3alpha,17beta-diol + NAD+ | - |
? | |
estrone + NADPH + H+ | - |
Homo sapiens | 17beta-estradiol + NADP+ | - |
? | |
testosterone + NAD+ | - |
Homo sapiens | androst-4-ene-3,17-dione + NADH + H+ | - |
? | |
testosterone + NAD+ | - |
Homo sapiens | DELTA4-3,17-androstendione + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AKR1B15 | - |
Homo sapiens |
aldo-keto reductase 1B15 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
17beta-estradiol | pH 7.4, 37°C recombinant His-tagged enzyme | Homo sapiens | |
0.6 | - |
testosterone | pH 7.4, 37°C recombinant His-tagged enzyme | Homo sapiens | |
1 | - |
estrone | pH 7.4, 37°C recombinant His-tagged enzyme | Homo sapiens | |
1.1 | - |
DELTA4-3,17-androstendione | pH 7.4, 37°C recombinant His-tagged enzyme | Homo sapiens | |
1.7 | - |
androsterone | pH 7.4, 37°C recombinant His-tagged enzyme | Homo sapiens | |
3 | - |
5alpha-androstane 3alpha,17beta-diol | pH 7.4, 37°C recombinant His-tagged enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | AKR1B15.1 is a mitochondrial carbonyl reductase. Two alternatively spliced protein isoforms encoded by the human AKRgene AKR1B15 exist, the AKR1B15.1 isoform catalyzes reduction of steroids and 3-keto-acyl-CoA conjugates | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
17beta-estradiol | pH 7.4, 37°C recombinant His-tagged enzyme | Homo sapiens | |
1.5 | - |
testosterone | pH 7.4, 37°C recombinant His-tagged enzyme | Homo sapiens | |
2.67 | - |
5alpha-androstane 3alpha,17beta-diol | pH 7.4, 37°C recombinant His-tagged enzyme | Homo sapiens | |
6.33 | - |
estrone | pH 7.4, 37°C recombinant His-tagged enzyme | Homo sapiens | |
10 | - |
DELTA4-3,17-androstendione | pH 7.4, 37°C recombinant His-tagged enzyme | Homo sapiens | |
11 | - |
androsterone | pH 7.4, 37°C recombinant His-tagged enzyme | Homo sapiens |