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Literature summary for 1.1.1.62 extracted from

  • Weber, S.; Salabei, J.K.; Moeller, G.; Kremmer, E.; Bhatnagar, A.; Adamski, J.; Barski, O.A.
    Aldo-keto Reductase 1B15 (AKR1B15): a mitochondrial human aldo-keto reductase with activity toward steroids and 3-keto-acyl-CoA conjugates (2015), J. Biol. Chem., 290, 6531-6545.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.62

Cloned(Commentary)

Cloned (Comment) Organism
gene AKR1B15 located on chromosome 7, cloning of two isoforms: AKR1B15.1 and AKR1B15.2, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged isozymes in Escherichia coli strain BL21(DE3), recombinant expression of C- or N-terminally c-Myc-tagged isozymes in HEK-293 cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics, binding of dinucleotide cofactors to AKR1B15.1, overview Homo sapiens
0.0019
-
 DELTA4-3,17-androstendione pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
0.0025
-
 estrone pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
0.0028
-
 androsterone pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
0.0071
-
 testosterone pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
0.0091
-
 17beta-estradiol pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
0.0192
-
 5alpha-androstane 3alpha,17beta-diol pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol isozyme AKR1B15.2 Homo sapiens 5829
-
mitochondrion isozyme AKR1B15.1 Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
17beta-estradiol + NADP+ Homo sapiens
-
 estrone + NADPH + H+
-
 ?
5alpha-androstane-3alpha,17beta-diol + NAD+ Homo sapiens
-
 androsterone + NADH + H+
-
 ?
testosterone + NAD+ Homo sapiens
-
 DELTA4-3,17-androstendione + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens C9JRZ8
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged isozymes AKR1B15.1 and AKR1B15.2 isozymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
adipose tissue isozymes AKR1B15.1 and AKR1B15.2 Homo sapiens
-
brain fetal, very low expression of isozyme AKR1B15.2 Homo sapiens
-
heart very low expression of isozymes AKR1B15.1 and AKR1B15.2 Homo sapiens
-
HEK-293 cell isozymes AKR1B15.1 and AKR1B15.2 Homo sapiens
-
additional information isozyme tissue-specific expression analysis, overview Homo sapiens
-
ovary low expression of isozymes AKR1B15.1 and AKR1B15.2 Homo sapiens
-
placenta isozymes AKR1B15.1 and AKR1B15.2 Homo sapiens
-
prostate isozyme AKR1B15.1 Homo sapiens
-
skeletal muscle isozymes AKR1B15.1 and AKR1B15.2 Homo sapiens
-
testis isozyme AKR1B15.1, very low expression of isozyme AKR1B15.2 Homo sapiens
-
thymus isozymes AKR1B15.1 and AKR1B15.2 Homo sapiens
-
thyroid gland isozyme AKR1B15.2 Homo sapiens
-
uterus very low expression of isozymes AKR1B15.1 and AKR1B15.2 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
17beta-estradiol + NADP+
-
 Homo sapiens estrone + NADPH + H+
-
 ?
5alpha-androstane-3alpha,17beta-diol + NAD+
-
 Homo sapiens androsterone + NADH + H+
-
 ?
androst-4-ene-3,17-dione + NADH + H+
-
 Homo sapiens testosterone + NAD+
-
 ?
androsterone + NADH + H+
-
 Homo sapiens 5alpha-androstane-3alpha,17beta-diol + NAD+
-
 ?
estrone + NADPH + H+
-
 Homo sapiens 17beta-estradiol + NADP+
-
 ?
testosterone + NAD+
-
 Homo sapiens androst-4-ene-3,17-dione + NADH + H+
-
 ?
testosterone + NAD+
-
 Homo sapiens DELTA4-3,17-androstendione + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
AKR1B15
-
 Homo sapiens
aldo-keto reductase 1B15
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
 17beta-estradiol pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
0.6
-
 testosterone pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
1
-
 estrone pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
1.1
-
 DELTA4-3,17-androstendione pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
1.7
-
 androsterone pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
3
-
 5alpha-androstane 3alpha,17beta-diol pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Homo sapiens
NADPH
-
 Homo sapiens

General Information

General Information Comment Organism
metabolism AKR1B15.1 is a mitochondrial carbonyl reductase. Two alternatively spliced protein isoforms encoded by the human AKRgene AKR1B15 exist, the AKR1B15.1 isoform catalyzes reduction of steroids and 3-keto-acyl-CoA conjugates Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1
-
 17beta-estradiol pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
1.5
-
 testosterone pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
2.67
-
 5alpha-androstane 3alpha,17beta-diol pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
6.33
-
 estrone pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
10
-
 DELTA4-3,17-androstendione pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
11
-
 androsterone pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens