Cloned (Comment) | Organism |
---|---|
expressed as GST-fusion proteins in Escherichia coli JM107 cells | Curvularia lunata |
Protein Variants | Comment | Organism |
---|---|---|
H164G | mutation results in a conformation of the enzyme that possesses a higher catalytic activity | Curvularia lunata |
H164W | mutation results in a conformation of the enzyme that possesses a higher catalytic activity | Curvularia lunata |
V161G | mutation increases the initial rates for the conversion of androstenedione to testosterone | Curvularia lunata |
Y212A | mutation increases the initial rates for the conversion of androstenedione to testosterone | Curvularia lunata |
Y49D | mutation changes the coenzyme specificity of the enzyme. The mutant can convert the steroids not only in the presence of NADP+ and NADPH, but also in the presence of NADH and NAD+ | Curvularia lunata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000088 | - |
NADP+ | 25°C, pH 8.0, wild-type enzyme, cosubstrate: 4-estrene-17beta-ol-3-one | Curvularia lunata | |
0.00019 | - |
NADP+ | 25°C, pH 8.0, mutant enzyme H164W, cosubstrate: 4-estrene-17beta-ol-3-one | Curvularia lunata | |
0.00028 | - |
NADP+ | 25°C, pH 8.0, mutant enzyme H164G, cosubstrate: 4-estrene-17beta-ol-3-one | Curvularia lunata | |
0.00035 | - |
NADPH | 25°C, pH 8.0, mutant enzyme H164G, cosubstrate: 4-estrene-3,17-dione | Curvularia lunata | |
0.0004 | - |
NADPH | 25°C, pH 8.0, mutant enzyme H164W, cosubstrate: 4-estrene-3,17-dione | Curvularia lunata | |
0.0065 | - |
NADPH | 25°C, pH 8.0, wild-type enzyme, cosubstrate: 4-estrene-3,17-dione | Curvularia lunata | |
0.16 | - |
NADPH | 25°C, pH 8.0, mutant enzyme Y49D, cosubstrate: 4-estrene-3,17-dione | Curvularia lunata | |
0.5 | - |
NAD+ | 25°C, pH 8.0, mutant enzyme Y49D, cosubstrate: 4-estrene-17beta-ol-3-one | Curvularia lunata | |
0.86 | - |
NADH | 25°C, pH 8.0, mutant enzyme Y49D, cosubstrate: 4-estrene-3,17-dione | Curvularia lunata | |
9 | - |
NADP+ | 25°C, pH 8.0, mutant enzyme Y49D, cosubstrate: 4-estrene-17beta-ol-3-one | Curvularia lunata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Curvularia lunata | O93874 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-estrene-3,17-dione + NADH + H+ | - |
Curvularia lunata | 4-estrene-17beta-ol-3-one + NAD+ | - |
r | |
4-estrene-3,17-dione + NADPH + H+ | - |
Curvularia lunata | 4-estrene-17beta-ol-3-one + NADP+ | - |
r | |
5alpha-androstane-3,17-dione + NADH + H+ | - |
Curvularia lunata | 5alpha-androstane-17beta-ol-3-one + NAD+ | - |
r | |
androst-4-ene-3,17-dione + NADH + H+ | - |
Curvularia lunata | testosterone + NAD+ | - |
r | |
estrone + NADH + H+ | - |
Curvularia lunata | estradiol + NAD+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
17beta-HSDcl | - |
Curvularia lunata |
17beta-hydroxysteroid dehydrogenase | - |
Curvularia lunata |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.065 | - |
NADPH | 25°C, pH 8.0, mutant enzyme Y49D, cosubstrate: 4-estrene-3,17-dione | Curvularia lunata | |
0.24 | - |
NAD+ | 25°C, pH 8.0, mutant enzyme Y49D, cosubstrate: 4-estrene-17beta-ol-3-one | Curvularia lunata | |
0.27 | - |
NADH | 25°C, pH 8.0, mutant enzyme Y49D, cosubstrate: 4-estrene-3,17-dione | Curvularia lunata | |
0.54 | - |
NADP+ | 25°C, pH 8.0, mutant enzyme Y49D, cosubstrate: 4-estrene-17beta-ol-3-one | Curvularia lunata | |
0.65 | - |
NADP+ | 25°C, pH 8.0, wild-type enzyme, cosubstrate: 4-estrene-17beta-ol-3-one | Curvularia lunata | |
1.8 | - |
NADPH | 25°C, pH 8.0, mutant enzyme H164W, cosubstrate: 4-estrene-3,17-dione | Curvularia lunata | |
1.9 | - |
NADPH | 25°C, pH 8.0, mutant enzyme H164G, cosubstrate: 4-estrene-3,17-dione | Curvularia lunata | |
3.1 | - |
NADP+ | 25°C, pH 8.0, mutant enzyme H164W, cosubstrate: 4-estrene-17beta-ol-3-one | Curvularia lunata | |
3.8 | - |
NADPH | 25°C, pH 8.0, wild-type enzyme, cosubstrate: 4-estrene-3,17-dione | Curvularia lunata | |
4.7 | - |
NADP+ | 25°C, pH 8.0, mutant enzyme H164G, cosubstrate: 4-estrene-17beta-ol-3-one | Curvularia lunata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | wild-type enzyme shows no activity with NADP+ | Curvularia lunata | |
NADH | wild-type enzyme shows no activity with NADPH | Curvularia lunata | |
NADP+ | mutant enzyme Y49D show activity with NADP+, wild-type enzyme shows no activity | Curvularia lunata | |
NADPH | mutant enzyme Y49D show activity with NADPH, wild-type enzyme shows no activity | Curvularia lunata |