Protein Variants | Comment | Organism |
---|---|---|
A170E/F172E | no detectable activity | Homo sapiens |
K159A | almost inactive enzyme, catalytic efficiencies of the substituted enzyme are more than 200fold lower for both reduction and oxidation compared with the values measured for the wild type enzyme | Homo sapiens |
L111E/V113F | no detectable activity | Homo sapiens |
additional information | deleting the last 36 amino acids does not have a dramatic effect on the catalytic properties | Homo sapiens |
S142A | almost inactive enzyme, catalytic efficiencies of the substituted enzyme are more than 200fold lower for both reduction and oxidation compared with the values measured for the wild type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-values for mutant enzymes in cell homogenate | Homo sapiens | |
0.0021 | - |
estradiol-17beta | wild-type enzyme | Homo sapiens | |
0.00253 | - |
estradiol-17beta | mutant enzyme SS134A | Homo sapiens | |
0.78 | - |
estrone | mutant enzyme S134A | Homo sapiens | |
0.9 | - |
estrone | wilde-type enzyme | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | 78000 | non-denaturing PAGE, gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
estrone + NADH | Homo sapiens | type 1 enzyme, catalyzes the conversion of the low activity estrogen, estrone, into highly active estradiol, both in the gonads and in target tissues | estradiol-17beta + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
type 1 enzyme | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | enzyme is phosphorylated by protein kinase A exclusively at Ser134 in vitro, but this phosphorylation has no effect on the catalytic properties of the type 1 enzyme, and most likely it does not occur in vivo | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
estradiol-17beta + NAD+ | - |
Homo sapiens | estrone + NADH | - |
? | |
estrone + NADH | - |
Homo sapiens | estradiol-17beta + NAD+ | - |
? | |
estrone + NADH | type 1 enzyme, catalyzes the conversion of the low activity estrogen, estrone, into highly active estradiol, both in the gonads and in target tissues | Homo sapiens | estradiol-17beta + NAD+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | cofactor | Homo sapiens | |
NADH | cofactor | Homo sapiens |