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Literature summary for 1.1.1.61 extracted from
- Reliable, sensitive, rapid and quantitative enzyme-based assay for gamma-hydroxybutyric acid (GHB) (2004), J. Forensic Sci., 49, 379-387.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | dipstick assay for the detection of gamma-hydroxybutyrate in alcoholic beverages | Cupriavidus necator |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli pLysS as stable fusion protein | Cupriavidus necator |
General Stability
| General Stability | Organism |
|---|---|
| addition of ammonium sulfate, glycerol, 2-mercaptoethanol, dithiothreitol, glutathione, or EDTA reduces activity during storage | Cupriavidus necator |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.19 | - |
gamma-hydroxybutyrate | pH 8.5, 23°C | Cupriavidus necator |  |
| 14 | - |
ethanol | pH 8.5, 23°C | Cupriavidus necator | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40500 | - |
calculated from the predicted amino acid sequence of the native enzyme | Cupriavidus necator |
| 66000 | - |
fusion protein, SDS-PAGE, calculated mass | Cupriavidus necator |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-hydroxybutanoate + NADP+ | Cupriavidus necator | - |
succinate semialdehyde + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cupriavidus necator | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cupriavidus necator |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-hydroxybutanoate + NADP+ | - |
Cupriavidus necator | succinate semialdehyde + NADPH + H+ | - |
? | |
| ethanol + NADP+ | - |
Cupriavidus necator | ethanal + NADPH + H+ | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| -80 | - |
stable for indefinite timespan | Cupriavidus necator |
| 4 | - |
stable for at least 21 days, 60% of activity after 3.5 months | Cupriavidus necator |
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