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Literature summary for 1.1.1.6 extracted from
- Glycerol dehydrogenase from Cellulomonas sp. NT 3060: purification and characterization (1982), Agric. Biol. Chem., 46, 2333-2339.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NH4+ | oxidation and reduction greatly increased | Cellulomonas sp. |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 1 mM | Cellulomonas sp. | |
| Cd2+ | strongly inhibitory | Cellulomonas sp. | |
| Cu2+ | strong inhibition | Cellulomonas sp. | |
| dihydroxyacetone | above 0.5 mM | Cellulomonas sp. | |
| Fe3+ | - |
Cellulomonas sp. | |
| K+ | reduction of dihydroxyacetone | Cellulomonas sp. | |
| p-chloromercuribenzoate | - |
Cellulomonas sp. | |
| sodium tetraborate | 1 mM, 53% inhibition | Cellulomonas sp. | |
| Zn2+ | strong inhibitory | Cellulomonas sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.089 | - |
NAD+ | pH 9.0, 25°C | Cellulomonas sp. | |
| 0.385 | - |
dihydroxyacetone | pH 6.0, 25°C | Cellulomonas sp. | |
| 10.9 | - |
glycerol | pH 9.0, 25°C | Cellulomonas sp. | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | soluble, isolation from crude cell extract | Cellulomonas sp. | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | oxidation of glycerol os greatly increased | Cellulomonas sp. | |
| Li+ | oxidation slightly increased | Cellulomonas sp. | |
| Na+ | oxidation and reduction slightly increased | Cellulomonas sp. | |
| Rb+ | oxidation greatly increased | Cellulomonas sp. | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerol + NAD+ | Cellulomonas sp. | - |
dihydroxyacetone + NADH + H+ | - |
? | |
| glycerol + NAD+ | Cellulomonas sp. NT3060 | - |
dihydroxyacetone + NADH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cellulomonas sp. | - |
- |
- |
| Cellulomonas sp. NT3060 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cellulomonas sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 138 | - |
- |
Cellulomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-propanediol + NAD+ | relative activity is 116% compared to oxidation of glycerol | Cellulomonas sp. | hydroxyacetone + NADH | - |
? | |
| 1,2-propanediol + NAD+ | relative activity is 116% compared to oxidation of glycerol | Cellulomonas sp. NT3060 | hydroxyacetone + NADH | - |
? | |
| 1,3-butanediol + NAD+ | relative activity is 6.5% compared to oxidation of glycerol | Cellulomonas sp. | ? | - |
? | |
| 1,3-propanediol + NAD+ | relative rate of oxidation is 18% compared to oxidation of glycerol | Cellulomonas sp. | ? | - |
? | |
| 1,4-butanediol + NAD+ | relative activity is 0.3% compared to oxidation of glycerol | Cellulomonas sp. | ? | - |
? | |
| 2,3-butanediol + NAD+ | - |
Cellulomonas sp. | 3-hydroxybutane-2-one + NADH | - |
? | |
| 2,3-butanediol + NAD+ | - |
Cellulomonas sp. NT3060 | 3-hydroxybutane-2-one + NADH | - |
? | |
| DL-alpha-glycerophosphate + NAD+ | oxidation at 16% compared to oxidation of glycerol | Cellulomonas sp. | ? | - |
? | |
| DL-glyceraldehyde + NAD+ | - |
Cellulomonas sp. | 3-hydroxypyruvaldehyde + NADH | - |
? | |
| ethanediol + NAD+ | - |
Cellulomonas sp. | glycolaldehyde + NADH | reduction at concentration of 50 mM | r | |
| ethanediol + NAD+ | - |
Cellulomonas sp. NT3060 | glycolaldehyde + NADH | reduction at concentration of 50 mM | r | |
| ethylene glycol + NAD+ | - |
Cellulomonas sp. | ? | - |
? | |
| glycerol + NAD+ | - |
Cellulomonas sp. | dihydroxyacetone + NADH + H+ | - |
? | |
| glycerol + NAD+ | - |
Cellulomonas sp. | dihydroxyacetone + NADH + H+ | - |
r | |
| glycerol + NAD+ | - |
Cellulomonas sp. NT3060 | dihydroxyacetone + NADH + H+ | - |
? | |
| glycerol + NAD+ | - |
Cellulomonas sp. NT3060 | dihydroxyacetone + NADH + H+ | - |
r | |
| glycerol-alpha-monochlorohydrin + NAD+ | relative rate of oxidation is 71% compared to oxidation of glycerol | Cellulomonas sp. | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
oxidation of glycerol | Cellulomonas sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
60 min, stable in the pH range pH 5.5-10 | Cellulomonas sp. |
| 50 | - |
15 min, stable around pH 7.0 | Cellulomonas sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
reaction of dihydroxyacetone | Cellulomonas sp. |
| 9 | - |
oxidation of glycerol | Cellulomonas sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | 7.8 | pH 4.8: about 40% of maximal activity, pH 7.8: about 50% of maximal activity, reduction of dihydroxyacetone | Cellulomonas sp. |
| 7 | 10 | pH 7.0: about 60% of maximal activity, pH 10.0: about 85% of maximal activity, oxidation of glycerol | Cellulomonas sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 10 | 30°C, 60 min, stable | Cellulomonas sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Cellulomonas sp. | |
| NADH | - |
Cellulomonas sp. | |
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