Activating Compound | Comment | Organism | Structure |
---|---|---|---|
citrate | the enzyme is more active in presence of multivalent anions, citrate, phosphate or sulfate, in comparison to monovalent anions, e.g. acetate or chloride | Pichia membranifaciens | |
phosphate | the enzyme is more active in presence of multivalent anions, citrate, phosphate or sulfate, in comparison to monovalent anions, e.g. acetate or chloride | Pichia membranifaciens | |
SO42- | the enzyme is more active in presence of multivalent anions, citrate, phosphate or sulfate, in comparison to monovalent anions, e.g. acetate or chloride | Pichia membranifaciens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-amino-2-(hydroxymethyl)-1,3-propanediol | strong inhibition, IC50 2 mM | Pichia membranifaciens | |
2-mercaptoethanol | strong inhibition, IC50 0.3 mM | Pichia membranifaciens | |
dihydroxyacetone phosphate | - |
Pichia membranifaciens | |
dithiothreitol | slight inhibition | Pichia membranifaciens | |
ethylene glycol | strong inhibition, IC50 4 mM | Pichia membranifaciens | |
glycerol 3-phosphate | - |
Pichia membranifaciens | |
L-cysteine | slight inhibition | Pichia membranifaciens | |
N-ethylmaleimide | rapid inactivation, IC50: 0.2 mM | Pichia membranifaciens | |
NAD+ | - |
Pichia membranifaciens | |
NADH | product inhibition , competitive with NAD+ | Pichia membranifaciens | |
NADP+ | - |
Pichia membranifaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.048 | - |
NADH | 25°C | Pichia membranifaciens | |
0.14 | - |
NAD+ | 25°C | Pichia membranifaciens | |
0.77 | - |
dihydroxyacetone | 25°C | Pichia membranifaciens | |
58 | - |
glycerol | 25°C | Pichia membranifaciens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | soluble, isolation from crude cell extract | Pichia membranifaciens | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
2 * 38000, SDS-PAGE | Pichia membranifaciens |
76000 | 80000 | gel filtration, PAGE under nondenaturing conditions | Pichia membranifaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol + NAD+ | Pichia membranifaciens | - |
dihydroxyacetone + NADH + H+ | - |
? | |
glycerol + NAD+ | Pichia membranifaciens H 112 | - |
dihydroxyacetone + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pichia membranifaciens | - |
- |
- |
Pichia membranifaciens H 112 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pichia membranifaciens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
448 | - |
- |
Pichia membranifaciens |
Storage Stability | Organism |
---|---|
-15°C, with 5% culture filtrate or 5% boiled crude cell extract, 10% Ficoll, pH 7.5, stable for 1 month | Pichia membranifaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-propanediol + NAD+ | - |
Pichia membranifaciens | hydroxyacetone + NADH | - |
? | |
1,2-propanediol + NAD+ | - |
Pichia membranifaciens H 112 | hydroxyacetone + NADH | - |
? | |
DL-glyceraldehyde + NAD+ | - |
Pichia membranifaciens | 3-hydroxypyruvaldehyde + NADH | - |
? | |
DL-glyceraldehyde + NAD+ | - |
Pichia membranifaciens H 112 | 3-hydroxypyruvaldehyde + NADH | - |
? | |
glycerol + NAD+ | - |
Pichia membranifaciens | dihydroxyacetone + NADH + H+ | - |
? | |
glycerol + NAD+ | - |
Pichia membranifaciens H 112 | dihydroxyacetone + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38000, SDS-PAGE | Pichia membranifaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
36 | - |
- |
Pichia membranifaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | reduction of dihydroxyacetone, phosphate buffer | Pichia membranifaciens |
8 | - |
oxidation of glycerol, tetraethyl ammonium chloride/tetraethylammonium hydroxide buffer | Pichia membranifaciens |
9.5 | 10.5 | oxidation of glycerol, diphosphate buffer | Pichia membranifaciens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
most stable | Pichia membranifaciens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pichia membranifaciens | |
NADH | - |
Pichia membranifaciens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
NADH | 25°C | Pichia membranifaciens | |
1.09 | - |
NAD+ | 25°C | Pichia membranifaciens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.2 | - |
rapid inactivation, IC50: 0.2 mM | Pichia membranifaciens | N-ethylmaleimide | |
0.3 | - |
strong inhibition, IC50 0.3 mM | Pichia membranifaciens | 2-mercaptoethanol | |
2 | - |
strong inhibition, IC50 2 mM | Pichia membranifaciens | 2-amino-2-(hydroxymethyl)-1,3-propanediol | |
4 | - |
strong inhibition, IC50 4 mM | Pichia membranifaciens | ethylene glycol |