Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-methyl-2-buten-1-al | product inhibition, therefore the product must be removed via reaction with hydrazine during the enzyme assay | Pseudomonas putida | |
dithiothreitol | - |
Pseudomonas putida | |
EDTA | only at concentration of 100 mM 50% reduction | Pseudomonas putida | |
N-ethylmaleimide | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
benzyl alcohol | - |
Pseudomonas putida | |
0.007 | - |
3-chloro-2-buten-1-ol | - |
Pseudomonas putida | |
0.011 | - |
3-aminobenzyl alcohol | - |
Pseudomonas putida | |
0.012 | - |
(R)-(+)-perillyl alcohol | - |
Pseudomonas putida | |
0.036 | - |
cinnamyl alcohol | - |
Pseudomonas putida | |
0.04 | - |
3-methyl-2-buten-1-ol | - |
Pseudomonas putida | |
0.044 | - |
2-Buten-1-ol | - |
Pseudomonas putida | |
0.064 | - |
NAD+ | with 3-methyl-2-buten-1-ol | Pseudomonas putida | |
0.118 | - |
NAD+ | with benzyl alcohol | Pseudomonas putida | |
0.27 | - |
3-methyl-1-butanol | - |
Pseudomonas putida | |
1.1 | - |
1-butanol | - |
Pseudomonas putida | |
1.7 | - |
3-methyl-3-buten-1-ol | - |
Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | is not sensitive against metal ion chelators | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37700 | - |
4 * 37700, SDS-PAGE | Pseudomonas putida |
151000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methyl-2-buten-1-ol + NAD+ | Pseudomonas putida | can use substrate as sole carbon source | 3-methyl-2-buten-1-al + NADH | - |
? | |
3-methyl-2-buten-1-ol + NAD+ | Pseudomonas putida MB-1 | can use substrate as sole carbon source | 3-methyl-2-buten-1-al + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida MB-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
allyl alcohol + NADP+ = acrolein + NADPH + H+ | 3-methyl-2-buten-1-ol-dependent inducible activity | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.05 | - |
purified enzyme, substrate 1-butanol | Pseudomonas putida |
0.08 | - |
purified enzyme, substrate 3-methyl-3-buten-1-ol | Pseudomonas putida |
0.2 | - |
purified enzyme, substrate 3-methyl-1-butanol | Pseudomonas putida |
3.7 | - |
purified enzyme, substrate cinnamyl alcohol | Pseudomonas putida |
4.6 | - |
purified enzyme, substrate 2-buten-1-ol | Pseudomonas putida |
7.7 | - |
purified enzyme, substrate 3-methyl-2-buten-1-ol | Pseudomonas putida |
8.8 | - |
purified enzyme, substrate (R)-(+)-perillyl alcohol | Pseudomonas putida |
16 | - |
purified enzyme, substrate 3-chlor-2-buten-1-ol | Pseudomonas putida |
19.3 | - |
purified enzyme, in glycine buffer pH 9.4 | Pseudomonas putida |
22 | - |
purified enzyme, substrate benzyl alcohol | Pseudomonas putida |
38 | - |
purified enzyme, substrate aromatic 3-aminobenzyl alcohol | Pseudomonas putida |
38.8 | - |
purified enzyme, in bis-Tris-propane-hydrazine buffer pH 9.4 | Pseudomonas putida |
Storage Stability | Organism |
---|---|
-70°C, 50-65% ethylene glycol (v/v), frozen in liquid nitrogen, stable for several month | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-(+)-perillyl alcohol + NADP+ | - |
Pseudomonas putida | ? | - |
r | |
1-butanol + NADP+ | - |
Pseudomonas putida | ? | - |
r | |
2-buten-1-ol + NADP+ | - |
Pseudomonas putida | ? | - |
r | |
2-buten-2-ol + NADPH | - |
Pseudomonas putida | but-2-enal | - |
? | |
2-buten-2-ol + NADPH | - |
Pseudomonas putida MB-1 | but-2-enal | - |
? | |
3-aminobenzyl alcohol + NADP+ | - |
Pseudomonas putida | ? | - |
r | |
3-chloro-2-buten-1-ol + NADP+ | - |
Pseudomonas putida | 3-chloro-2-butenaldehyde + NADPH | - |
? | |
3-chloro-2-buten-1-ol + NADP+ | - |
Pseudomonas putida MB-1 | 3-chloro-2-butenaldehyde + NADPH | - |
? | |
3-methyl-1-butanol + NADP+ | - |
Pseudomonas putida | ? | - |
r | |
3-methyl-2-buten-1-ol + NAD+ | - |
Pseudomonas putida | 3-methyl-2-buten-1-al + NADH | - |
? | |
3-methyl-2-buten-1-ol + NAD+ | can use substrate as sole carbon source | Pseudomonas putida | 3-methyl-2-buten-1-al + NADH | - |
? | |
3-methyl-2-buten-1-ol + NAD+ | - |
Pseudomonas putida MB-1 | 3-methyl-2-buten-1-al + NADH | - |
? | |
3-methyl-2-buten-1-ol + NAD+ | can use substrate as sole carbon source | Pseudomonas putida MB-1 | 3-methyl-2-buten-1-al + NADH | - |
? | |
3-methyl-3-buten-1-ol + NADP+ | - |
Pseudomonas putida | ? | - |
r | |
benzyl alcohol + NADP+ | - |
Pseudomonas putida | ? | - |
r | |
cinnamyl alcohol + NADP+ | - |
Pseudomonas putida | cinnamyl aldehyde + NADPH | - |
? | |
cinnamyl alcohol + NADP+ | - |
Pseudomonas putida MB-1 | cinnamyl aldehyde + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 37700, SDS-PAGE | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
for reduction | Pseudomonas putida |
10 | - |
for oxidation | Pseudomonas putida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9.4 | - |
stability at pH 9.4, 25°C much higher than at reactions optimal pH of 10.0 | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | exhibits less than 5% of the activity with NADP+ | Pseudomonas putida |