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Literature summary for 1.1.1.54 extracted from

  • Hirata, T.; Tamura, Y.; Yokobatake, N.; Shimoda, K.; Ashida, Y.
    A 38 kDa allylic alcohol dehydrogenase from the cultured cells of Nicotiana tabacum (2000), Phytochemistry, 55, 297-303.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
sequence homology with other NADPH-dependent oxidoreductases from plants Nicotiana tabacum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
2 * 37000, SDS-PAGE and amino acid sequence analysis Nicotiana tabacum
74000
-
gel filtration Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
allyl alcohol + NADP+ Nicotiana tabacum
-
acrolein + NADPH
-
r

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Nicotiana tabacum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.11
-
-
Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S,4S)-carveol + NADP+
-
Nicotiana tabacum (S)-carvone + NADPH
-
r
(S)-carvone + NADPH
-
Nicotiana tabacum (2S,4S)-carveol + NADP+
-
r
allyl alcohol + NADP+
-
Nicotiana tabacum acrolein + NADPH
-
r
allyl alcohol + NADP+ specific for the (S)-isomer of secondary allylic alcohols, several stereomers tested for activity in both reaction directions Nicotiana tabacum acrolein + NADPH
-
r

Subunits

Subunits Comment Organism
dimer 2 * 37000, SDS-PAGE and amino acid sequence analysis Nicotiana tabacum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
reduction of carbonyl group of (S)-carvone Nicotiana tabacum
8
-
dehydrogenation of (S)-carveol Nicotiana tabacum

Cofactor

Cofactor Comment Organism Structure
NAD(P)H
-
Nicotiana tabacum
NADP+ activity 4times greater than with NAD+ Nicotiana tabacum