Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.51 extracted from

  • Herl, V.; Frankenstein, J.; Meitinger, N.; Mueller-Uri, F.; Kreis, W.
    Delta5-3beta-hydroxysteroid dehydrogenase (3betaHSD) from Digitalis lanata. Heterologous expression and characterisation of the recombinant enzyme (2007), Planta Med., 73, 704-710.
    View publication on PubMed
Search for more literature for 1.1.1.51

Cloned(Commentary)

Cloned (Comment) Organism
cDNA is functionally expressed as a His-tagged fusion protein in Escherichia coli Digitalis lanata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 5-pregnen-3beta-ol-20-one 50°C, pH 7.8 Digitalis lanata
0.106
-
 5alpha-pregnan-3,20-dione 50°C, pH 7.8 Digitalis lanata
0.116
-
 5alpha-pregnan-3beta-ol-20-one 50°C, pH 7.8 Digitalis lanata
0.26
-
 NAD+ 50°C, pH 7.8 Digitalis lanata
0.314
-
 5beta-Pregnan-3beta-ol-20-one 50°C, pH 7.8 Digitalis lanata
0.642
-
 NADH 50°C, pH 7.8 Digitalis lanata
0.642
-
 5-androsten-3beta-ol-17-one 50°C, pH 7.8 Digitalis lanata
1.725
-
 5beta-pregnan-3,20-dione 50°C, pH 7.8 Digitalis lanata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28561
-
 x * 28561, calculated from sequence Digitalis lanata

Organism

Organism UniProt Comment Textmining
Digitalis lanata Q93Y47
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Digitalis lanata

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Digitalis lanata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-androsten-17beta-ol-3-one + NAD(P)+ 17keto function is reduced. NADP+ is 3-5times less effective than NAD+ Digitalis lanata 4-androstene-3,17-dione + NAD(P)H + H+
-
 ?
5-androsten-3beta-ol-17-one + NAD(P)+ NADP+ is 3-5times less effective than NAD+ Digitalis lanata 5-androstene-3,17-dione + NAD(P)H + H+
-
 ?
5-pregnen-3beta-ol-20-one + NAD(P)+ NADP+ is 3-5times less effective than NAD+ Digitalis lanata 5-pregnene-3,20-dione + NAD(P)H + H+
-
 ?
5alpha-pregnan-3beta-ol-20-one + NAD(P)+ NADP+ and NADPH are 3-5times less effective than NAD+ and NADH Digitalis lanata 5alpha-pregnan-3,20-dione + NAD(P)H + H+
-
 r
5alpha-pregnan-3beta-ol-20-one + NAD+
-
 Digitalis lanata 5alpha-pregnane-3,20-dione + NADH + H+
-
 r
5alpha-pregnane-3,20-dione + NADH + H+
-
 Digitalis lanata 5alpha-pregnan-3beta-ol-20-one + NAD+
-
 r
5beta-pregnan-3,20-dione + NAD(P)H + H+ NADP+ is 3-5times less effective than NAD+ Digitalis lanata 3beta-hydroxy-5beta-pregnan-20-one + NAD(P)+
-
 ?
5beta-pregnan-3beta-ol-20-one + NAD(P)+ NADP+ and NADPH are 3-5times less effective than NAD+ and NADH Digitalis lanata 5-pregnan-3,20-dione + NAD(P)H + H+
-
 r

Subunits

Subunits Comment Organism
? x * 28561, calculated from sequence Digitalis lanata

Synonyms

Synonyms Comment Organism
3betaHSD
-
 Digitalis lanata
DELTA5-3beta-hydroxysteroid dehydrogenase
-
 Digitalis lanata
pQ3 beta HSD
-
 Digitalis lanata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Digitalis lanata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 20 mM Tris-HCl Digitalis lanata

pI Value

Organism Comment pI Value Maximum pI Value
Digitalis lanata isoelectric focusing, pH-range: 3-9
-
 6.5