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Literature summary for 1.1.1.50 extracted from
- The Del1 deposition domain can immobilize 3alpha-hydroxysteroid dehydrogenase in the extracellular matrix without interfering with enzymatic activity (2009), Bioprocess Biosyst. Eng., 32, 569-573.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | transfection of cells with plasmids encoding a 3alpha-hydroxysteroid dehydrogenase-Del1 deposition domain fusion protein. The Del1 deposition domain immobilizes the enzyme in the extracellular matrix without interfering with its enzymatic activity. Extracellular matrix conditioned by cells transfected with 3alpha-hydroxysteroid dehydrogenase-Del1 deposition domain fusion significantly suppresses the growth of otherwise untreated LNCaP cells | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in COS-7 cells | Mus musculus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
x * 43000, 3alpha-hydroxysteroid dehydrogenase, x * 65000, 3alpha-hydroxysteroid dehydrogenase-Del1 deposition domain fusion protein, SDS-PAGE | Homo sapiens |
| 65000 | - |
x * 43000, 3alpha-hydroxysteroid dehydrogenase, x * 65000, 3alpha-hydroxysteroid dehydrogenase-Del1 deposition domain fusion protein, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | Q91WR5 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| LNCaP cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3alpha,17beta-androstene-3,17-diol + NAD(P)+ | - |
Mus musculus | dihydrotestosterone + NAD(P)H + H+ | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, 3alpha-hydroxysteroid dehydrogenase, x * 65000, 3alpha-hydroxysteroid dehydrogenase-Del1 deposition domain fusion protein, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3 alphaHD | - |
Mus musculus |
| 3alpha-hydroxysteroid dehydrogenase | - |
Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD(P)+ | - |
Mus musculus | |
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Release 2023.1 (January 2023)
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