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Literature summary for 1.1.1.50 extracted from

  • Lin, H.K.; Jez, J.M.; Schlegel, B.P.; Peehl, D.M.; Pachter, J.A.; Penning, T.M.
    Expression and characterization of recombinant type 2: 3alpha-hydroxysteroid dehydrogenase (HSD) from human prostate: demonstration of bifunctional 3alpha/17beta-HSD activity and cellular distribution (1997), Mol. Endocrinol., 11, 1971-1984.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of type II isoform in Escherichia coli Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
androstanedione NADPH, detection spectrophotometrically Homo sapiens
0.006
-
5alpha-dihydrotestosterone NADP+, detection radiometrically Homo sapiens
0.012
-
testosterone NADP+, detection radiometrically Homo sapiens
0.017
-
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide NADP+, detection spectrophotometrically Homo sapiens
0.018
-
5alpha-dihydrotestosterone NADPH, detection radiometrically Homo sapiens
0.019
-
5alpha-dihydrotestosterone NADPH, detection spectrophotometrically Homo sapiens
0.019
-
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide NADP+, detection radio- or fluorometrically Homo sapiens
0.021
-
9,10-phenanthrenequinone NADPH, detection spectrophotometrically Homo sapiens
0.048
-
androsterone NADP+, detection radiometrically Homo sapiens
0.087
-
4-nitrobenzaldehyde NADPH, detection spectrophotometrically Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens type 3 isoform in liver virtually identical with bile acid-binding protein ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
type 2 isoform
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from Escherichia coli Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ enzyme isoforms bind substrate in different ways Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
colon
-
Homo sapiens
-
heart
-
Homo sapiens
-
kidney
-
Homo sapiens
-
liver
-
Homo sapiens
-
lung
-
Homo sapiens
-
additional information type 2 isoform is expressed in several tissues Homo sapiens
-
ovary
-
Homo sapiens
-
prostate gland
-
Homo sapiens
-
skeletal muscle
-
Homo sapiens
-
small intestine
-
Homo sapiens
-
thymus
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0005
-
substrate androsterone with cofactor NADP+, detection radiometrically Homo sapiens
0.0013
-
substrate 5alpha-dihydrotestosterone with cofactor NADP+, detection radiometrically Homo sapiens
0.0025
-
substrate 5alpha-dihydrotestosterone with cofactor NADPH, detection spectrophotometrically Homo sapiens
0.0042
-
substrate 5alpha-dihydrotestosterone with cofactor NADPH, detection radiometrically Homo sapiens
0.0048
-
substrate 3alpha-androstanediol with cofactor NADP+, detection spectrophotometrically Homo sapiens
0.0085
-
substrate 3alpha-androstanediol with cofactor NADP+, detection radiometrically Homo sapiens
0.0111
-
substrate androstanedione with cofactor NADPH, detection spectrophotometrically Homo sapiens
0.0134
-
substrate 3alpha-androstanediol with cofactor NADP+, detection fluorometrically Homo sapiens
0.13
-
substrate 4-nitrobenzaldehyde with cofactor NADPH, detection spectrophotometrically Homo sapiens
0.538
-
substrate 9,10-phenanthrenequinone with cofactor NADPH, detection spectrophotometrically Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide + NADP+
-
Homo sapiens ?
-
?
5alpha-androstan-3alpha,17beta-diol + NAD(P)+
-
Homo sapiens 17beta-hydroxy-5alpha-androstan-3-one + NADPH
-
r
5alpha-androstan-3alpha,17beta-diol + NAD(P)H
-
Homo sapiens 5alpha-dihydrotestosterone + NAD(P)+
-
r
5alpha-dihydrotestosterone + NAD(P)H + H+
-
Homo sapiens 5alpha-androstan-3alpha,17beta-diol + NAD(P)+
-
r
5alpha-dihydrotestosterone + NAD(P)H + H+
-
Homo sapiens (3beta,5alpha,17beta)-androstane-3,17-diol + NAD(P)+
-
r
5beta-androstan-3,17-dione + NAD(P)H
-
Homo sapiens 5beta-androstan-17one-3-ol + NAD(P)+
-
r
5beta-androstan-3alpha,17beta-diol + NADP+
-
Homo sapiens 17beta-hydroxy-5beta-androstan-3-one + NADPH
-
r
9,10-phenanthrenequinone + NADPH
-
Homo sapiens ?
-
?
androsterone + NAD(P)+
-
Homo sapiens 5alpha-androstan-3,17-dione + NAD(P)H
-
r
androsterone + NADP+
-
Homo sapiens androstane-3,17-dione + NADPH
-
?
additional information can not catalyze conversion of 3alpha-androstanediol to 5alpha-dihydrotestosterone Homo sapiens ?
-
?
additional information type 3 isoform in liver virtually identical with bile acid-binding protein Homo sapiens ?
-
?
p-nitrobenzaldehyde + NAD(P)H
-
Homo sapiens p-nitrobenzylalcohol + NAD(P)+
-
?
testosterone + NAD(P)+
-
Homo sapiens androstenedione + NAD(P)H + H+
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00783
-
androsterone
-
Homo sapiens
0.0398
-
5alpha-dihydrotestosterone detection fluorometrically Homo sapiens
0.0757
-
testosterone
-
Homo sapiens
0.0965
-
5alpha-dihydrotestosterone detection spectrophotometrically Homo sapiens
0.114
-
5alpha-dihydrotestosterone detection radiometrically Homo sapiens
0.203
-
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide detection spectrophotometrically Homo sapiens
0.32
-
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide detection radiometrically Homo sapiens
0.507
-
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide detection fluorometrically Homo sapiens
1.08
-
4-nitrobenzaldehyde
-
Homo sapiens
1.9
-
androstanedione detection spectrophotometrically Homo sapiens
18.9
-
9,10-phenanthrenequinone
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Homo sapiens
NADPH
-
Homo sapiens