Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Camelus dromedarius |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Camelus dromedarius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
x * 63000, SDS-PAGE | Camelus dromedarius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | Camelus dromedarius | - |
D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Camelus dromedarius | G1EHI3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme 95.5fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Camelus dromedarius |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
289.6 | - |
purified recombinant His-tagged enzyme, pH 7.5, 25°C | Camelus dromedarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | - |
Camelus dromedarius | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 63000, SDS-PAGE | Camelus dromedarius |
More | structure modeling | Camelus dromedarius |
Synonyms | Comment | Organism |
---|---|---|
G6PD | - |
Camelus dromedarius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Camelus dromedarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Camelus dromedarius |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | the enzyme has two NADP+ binding sites, one is the structural NADP+ and the second is the catalytic NADP+. The structural NADP+ domain adopt a classic beta-alpha-beta dinucleotide fold with the fingerprint sequence GASGDLA at the tight turn following the first beta-strand | Camelus dromedarius |