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Literature summary for 1.1.1.49 extracted from

  • Nee, G.; Zaffagnini, M.; Trost, P.; Issakidis-Bourguet, E.
    Redox regulation of chloroplastic glucose-6-phosphate dehydrogenase: a new role for f-type thioredoxin (2009), FEBS Lett., 583, 2827-2832.
    View publication on PubMed
Search for more literature for 1.1.1.49

Activating Compound

Activating Compound Comment Organism Structure
anhydrotetracyclin induces expression of mature G6PDH1 Arabidopsis thaliana
additional information oxidative activation is strictly dependent on plastidial thioredoxins that show differential efficiencies. Trx f1 regulates G6PDH1 activity as efficiently as Trx m1 or m4. Trx x is a very poor regulator of G6PDH activity. Trx y1 is inefficient as inhibitor but it shows high efficiency in activation. G6PDH activity can be recovered by transferring from light to dark, reversibility of inactivation certifying redox modulation of the enzyme. In the dark, reactivation can be enhanced by the addition of oxidants, such as oxidized dithiothreitol and H2O2 Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha strain from the pASK-G6PDH1 plasmid Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
additional information Trx f1 regulates G6PDH1 activity as efficiently as Trx m1 or m4. Trx x is a very poor regulator of G6PDH activity. Trx y1 is inefficient as inhibitor but it shows high efficiency in activation. Upon illumination, a strong and fast reductive inhibition of G6PDH1 activity dependent on the presence of all the components of the Fd/Trx system Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast stroma
-
 Arabidopsis thaliana 9570
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q43727
-
-

Purification (Commentary)

Purification (Comment) Organism
by affinity chromatography, to homogeneity Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.23
-
 purified G6PDH1 upon reduction by reduced DTT Arabidopsis thaliana
20.3
-
 purified G6PDH1 Arabidopsis thaliana
139.5
-
 purified G6PDH1 upon oxidation by diamide Arabidopsis thaliana

Storage Stability

Storage Stability Organism
-20°C, 100 mM Tris-HCl buffer, pH 7.9, several months Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 6-phosphate + NADP+
-
 Arabidopsis thaliana D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
G6PDH1
-
 Arabidopsis thaliana