Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | 2.0 mM, 65% inhibition, G6PDH-2; G6PDH-1 | Deinococcus radiophilus | |
beta-naphthoquinone-4-sulfonic acid | 1 mM, 68% inhibition, G6PDH-1; 1 mM, 81% inhibition, G6PDH-2 | Deinococcus radiophilus | |
Cd2+ | 1 mM, 53% inhibition, G6PDH-1 | Deinococcus radiophilus | |
Cu2+ | 1 mM, 24% inhibition, G6PDH-1; 1 mM, 78% inhibition, G6PDH-2 | Deinococcus radiophilus | |
EDTA | 5 mM, 85% inhibition, G6PDH-1; 5 mM, 97% inhibition, G6PDH-2 | Deinococcus radiophilus | |
Hg2+ | 1 mM, 47% inhibition, G6PDH-2; 1 mM, 84% inhibition, G6PDH-1 | Deinococcus radiophilus | |
oleic acid | G6PDH-1 is more susceptible to oleic acid than G6PDH-2 | Deinococcus radiophilus | |
Zn2+ | 1 mM, 83% inhibition, G6PDH-2; 1 mM, 94% inhibition, G6PDH-1 | Deinococcus radiophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, 1.6fold activation, G6PDH-2 | Deinococcus radiophilus | |
Cd2+ | 1 mM, 1.3fold activation, G6PDH-2 | Deinococcus radiophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35200 | - |
4 * 35200, G6PDH-1, SDS-PAGE | Deinococcus radiophilus |
60000 | - |
2 * 60000, G6PDH-2, SDS-PAGE | Deinococcus radiophilus |
110000 | - |
G6PDH-2, gel filtration | Deinococcus radiophilus |
130000 | - |
G6PDH-1, gel filtration | Deinococcus radiophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Deinococcus radiophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
G6PDH-1 | Deinococcus radiophilus |
G6PDH-2 | Deinococcus radiophilus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | G6PDH-2 is detected throughout the growth phase | Deinococcus radiophilus | - |
additional information | the enzyme is detected throughout the growth phase. The substantial increase in G6PDH-1 observed at stationary phase or as the results of external oxidative stress indicates that this enzyme is inducible under stressful environmental conditions | Deinococcus radiophilus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1033 | - |
cofactor: NADP+, G6PDH-2 | Deinococcus radiophilus |
1077 | - |
cofactor: NAD+, G6PDH-2 | Deinococcus radiophilus |
2890 | - |
cofactor: NADP+, G6PDH-1 | Deinococcus radiophilus |
3078 | - |
cofactor: NAD+, G6PDH-1 | Deinococcus radiophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NAD+ | - |
Deinococcus radiophilus | D-glucono-1,5-lactone 6-phosphate + NADH + H+ | - |
? | |
D-glucose 6-phosphate + NADP+ | - |
Deinococcus radiophilus | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 60000, G6PDH-2, SDS-PAGE | Deinococcus radiophilus |
tetramer | 4 * 35200, G6PDH-1, SDS-PAGE | Deinococcus radiophilus |
Synonyms | Comment | Organism |
---|---|---|
G6PDH-1 | - |
Deinococcus radiophilus |
G6PDH-2 | - |
Deinococcus radiophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
G6PDH-1 | Deinococcus radiophilus |
30 | - |
G6PDH-2 | Deinococcus radiophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
30 min, G6PDH-1 retains 40% of its activity | Deinococcus radiophilus |
70 | - |
30 min, G6PDH-2 retains 35% of its activity | Deinococcus radiophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
G6PDH-1 | Deinococcus radiophilus |
8 | - |
G6PDH-2 | Deinococcus radiophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dual coenzyme specificity for NAD+ and NADP+, G6PDH-1 | Deinococcus radiophilus | |
NAD+ | dual coenzyme specificity for NAD+ and NADP+, G6PDH-2 | Deinococcus radiophilus | |
NADP+ | dual coenzyme specificity for NAD+ and NADP+, G6PDH-1 | Deinococcus radiophilus | |
NADP+ | dual coenzyme specificity for NAD+ and NADP+, G6PDH-2 | Deinococcus radiophilus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Deinococcus radiophilus | isoelectric focusing, pH-range 3-10, G6PDH-2 | - |
5.7 |
Deinococcus radiophilus | isoelectric focusing, pH-range 3-10, G6PDH-1 | - |
6.4 |