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Literature summary for 1.1.1.49 extracted from
- Purification and properties of glucose 6-phosphate dehydrogenase from Aspergillus aculeatus (2005), J. Biochem. Mol. Biol., 38, 584-590.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Aspergillus aculeatus |  |
| ATP | - |
Aspergillus aculeatus | |
| Co2+ | competitive, 60% inhibition at 0.01 mM | Aspergillus aculeatus | |
| fructose 6-phosphate | - |
Aspergillus aculeatus | |
| additional information | product and dead-end inhibition studies, overview, no inhibition by AMP | Aspergillus aculeatus | |
| NADP+ | substrate inhibition | Aspergillus aculeatus | |
| NADPH | product inhibition | Aspergillus aculeatus | |
| phosphoenolpyruvate | - |
Aspergillus aculeatus | |
| Zn2+ | competitive, 40% inhibition at 0.01 mM | Aspergillus aculeatus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics, kinetic mechanism analysis, ternary-complex mechanism, overview | Aspergillus aculeatus | |
| 0.006 | - |
NADP+ | pH 7.5 | Aspergillus aculeatus | |
| 0.075 | - |
D-glucose 6-phosphate | pH 7.5 | Aspergillus aculeatus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Aspergillus aculeatus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
2 * 52000, SDS-PAGE | Aspergillus aculeatus |
| 105000 | - |
gel filtration | Aspergillus aculeatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + NADP+ | Aspergillus aculeatus | inhibition by divalent metal ions, at low NADPH /NADP+ ratio, is another means of controlling the pentose phosphate pathway | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus aculeatus | - |
filamentous fungus isolated from infected tongue of a human patient | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 230fold to homogeneity by ammonium sulfate fractionation, affinity chromatography, and gel filtration | Aspergillus aculeatus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | ternary-complex mechanism | Aspergillus aculeatus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Aspergillus aculeatus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 220 | - |
purified enzyme | Aspergillus aculeatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + NADP+ | inhibition by divalent metal ions, at low NADPH /NADP+ ratio, is another means of controlling the pentose phosphate pathway | Aspergillus aculeatus | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? | |
| D-glucose 6-phosphate + NADP+ | the substrate specificity is extremely strict | Aspergillus aculeatus | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? | |
| additional information | no activity with glucose and glucosamine 6-phosphate | Aspergillus aculeatus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 52000, SDS-PAGE | Aspergillus aculeatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| G6PD | - |
Aspergillus aculeatus |
| glucose 6-phosphate dehydrogenase | - |
Aspergillus aculeatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
purified enzyme, rapid and irreversible inactivation, half life: 3.2 min, in presence of substrate D-glucose 6-phosphate 7.5 min | Aspergillus aculeatus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 83 | - |
D-glucose 6-phosphate | pH 7.5 | Aspergillus aculeatus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Aspergillus aculeatus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | specific for, no activity with NAD+ | Aspergillus aculeatus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0047 | - |
Co2+ | pH 7.5 | Aspergillus aculeatus | |
| 0.0066 | - |
Zn2+ | pH 7.5 | Aspergillus aculeatus | |
| 0.02 | - |
NADPH | pH 7.5, versus NADP+ | Aspergillus aculeatus | |
| 0.023 | - |
NADP+ | pH 7.5 | Aspergillus aculeatus | |
| 0.041 | 0.043 | NADPH | pH 7.5, versus D-glucose 6-phosphate | Aspergillus aculeatus | |
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