KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | ping pong bi bi kinetic mechanism | Ovis aries | |
0.0147 | - |
NADP+ | pH 7.4, 37°C | Ovis aries | |
0.041 | - |
D-glucose 6-phosphate | pH 7.4, 37°C | Ovis aries |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Ovis aries | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ + H2O | Ovis aries | the enzyme is responsible for the production of NADPH, which is required for biosynthetic reactions and protection of cells from free radicals | D-gluconate 6-phosphate + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ovis aries | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 1384fold from kidney cortex by ultracentrifugation, 2',5'-ADP affinity and anion exchange chromatography | Ovis aries |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | the adult kidney cortex enzyme shows a ping pong bi bi kinetic mechanism, while the lamb kidney enzyme obeys an ordered bi bi mechanism, thus the kinetic mechanism alters due to aging | Ovis aries |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney cortex | - |
Ovis aries | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
27.69 | - |
purified kidney cortex enzyme | Ovis aries |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ + H2O | - |
Ovis aries | D-gluconate 6-phosphate + NADPH + H+ | - |
? | |
D-glucose 6-phosphate + NADP+ + H2O | the enzyme is responsible for the production of NADPH, which is required for biosynthetic reactions and protection of cells from free radicals | Ovis aries | D-gluconate 6-phosphate + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G-6-PD | - |
Ovis aries |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Ovis aries |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | temperature profile | Ovis aries |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Ovis aries |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | pH profile | Ovis aries |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10 | stable, purified enzyme | Ovis aries |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Ovis aries |