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Literature summary for 1.1.1.49 extracted from

  • Wang, X.; Lam, V.M.; Engel, P.C.
    Functional properties of two mutants of human glucose 6-phosphate dehydrogenase, R393G and R393H, corresponding to the clinical variants G6PD Wisconsin and Nashville (2006), Biochim. Biophys. Acta, 1762, 767-774.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Homo sapiens

Protein Variants

Protein Variants Comment Organism
R393E site-directed mutagenesis, the mutation affects a residue in the dimer interface close to the structural NADP+ site, the mutant activity is slightly reduced compared to the activity of the wild-type enzyme Homo sapiens
R393G naturally occuring mutation corresponding to the clinical variants G6PD Wisconsin, the mutation affects a residue in the dimer interface close to the structural NADP+ site, the mutant activity is similar to the activity of the wild-type enzyme Homo sapiens
R393H naturally occuring mutation corresponding to the clinical variants G6PD Nashville, the mutation affects a residue in the dimer interface close to the structural NADP+ site, the mutant activity is reduced compared to the activity of the wild-type enzyme Homo sapiens
R393I site-directed mutagenesis, the mutation affects a residue in the dimer interface close to the structural NADP+ site, the mutant activity is similar to the activity of the wild-type enzyme Homo sapiens
R393L site-directed mutagenesis, the mutation affects a residue in the dimer interface close to the structural NADP+ site, the mutant activity is similar to the activity of the wild-type enzyme Homo sapiens
R393V site-directed mutagenesis, the mutation affects a residue in the dimer interface close to the structural NADP+ site, the mutant activity is reduced compared to the activity of the wild-type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetics of wild-type and mutant enzymes Homo sapiens
0.0071
-
NADP+ pH 8.0, 25°C, recombinant wild-type enzyme Homo sapiens
0.0093
-
NADP+ pH 8.0, 25°C, recombinant mutant R393G Homo sapiens
0.0165
-
NADP+ pH 8.0, 25°C, recombinant mutant R393H Homo sapiens
0.052
-
D-glucose 6-phosphate pH 8.0, 25°C, recombinant wild-type enzyme Homo sapiens
0.067
-
D-glucose 6-phosphate pH 8.0, 25°C, recombinant mutant R393G Homo sapiens
0.19
-
D-glucose 6-phosphate pH 8.0, 25°C, recombinant mutant R393H Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose 6-phosphate + NADP+ Homo sapiens
-
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by 2',5'-ADP affinity chromatography Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Homo sapiens
130
-
purified recombinant mutant R393H Homo sapiens
135
-
purified recombinant mutant R393E Homo sapiens
166
-
purified recombinant mutant R393V Homo sapiens
176
-
purified recombinant mutant R393L Homo sapiens
178
-
purified recombinant mutant R393G Homo sapiens
180
-
purified recombinant wild-type enzyme Homo sapiens
181
-
purified recombinant mutant R393I Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 6-phosphate + NADP+
-
Homo sapiens D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
?

Synonyms

Synonyms Comment Organism
G6PD
-
Homo sapiens
glucose 6-phosphate dehydrogenase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
192
-
D-glucose 6-phosphate pH 8.0, 25°C, recombinant mutant R393H Homo sapiens
232
-
D-glucose 6-phosphate pH 8.0, 25°C, recombinant mutant R393G Homo sapiens
275
-
D-glucose 6-phosphate pH 8.0, 25°C, recombinant wild-type enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Homo sapiens