Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
4 * 55000, Entner-Doudoroff enzyme | Pseudomonas fluorescens |
65000 | - |
4 * 65000, Zwischenferment | Pseudomonas fluorescens |
220000 | - |
Entner-Doudoroff enzyme, gel chromatography | Pseudomonas fluorescens |
265000 | - |
Zwischenferment, gel chromatography | Pseudomonas fluorescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + H2O + NADP+ | Pseudomonas fluorescens | - |
D-gluconate 6-phosphate + NADPH + H+ | 6-phosphoglucono-delta-lactone | r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas fluorescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas fluorescens |
Storage Stability | Organism |
---|---|
4°C, several months | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + H2O + NADP+ | - |
Pseudomonas fluorescens | D-gluconate 6-phosphate + NADPH + H+ | 6-phosphoglucono-delta-lactone | r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 65000, Zwischenferment | Pseudomonas fluorescens |
tetramer | 4 * 55000, Entner-Doudoroff enzyme | Pseudomonas fluorescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas fluorescens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10.5 | stable at 5°C for 48 h | Pseudomonas fluorescens |
6.5 | 10 | Entner-Doudoroff enzyme stable | Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas fluorescens | |
NADP+ | - |
Pseudomonas fluorescens | |
NADPH | - |
Pseudomonas fluorescens |