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Literature summary for 1.1.1.47 extracted from
- Biochemical studies of enzymes involved in glycolysis of the thermoacidophilic crenarchaeon Sulfolobus solfataricus (2011), PH. D. Thesis Universität Duisburg-Essen, 2011, 0000.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Saccharolobus solfataricus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
NADP+ | cosubstrate D-glucose, 70°C, pH not specified in the publication | Saccharolobus solfataricus |  |
| 0.15 | - |
NADP+ | cosubstrate D-glucose, 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 0.15 | - |
NADP+ | cosubstrate D-glucose, 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 0.17 | - |
D-glucose | cosubstrate NADP+, 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 2.23 | - |
NAD+ | cosubstrate D-glucose, 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 3.3 | - |
NAD+ | cosubstrate D-glucose, 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 4.59 | - |
D-glucose | cosubstrate NAD+, 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 5.59 | - |
NAD+ | cosubstrate D-glucose, 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
x * 39000, SDS-PAGE, x * 39970, calculated | Saccharolobus solfataricus |
| 39970 | - |
x * 39000, SDS-PAGE, x * 39970, calculated | Saccharolobus solfataricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | Q97U21 | - |
- |
| Saccharolobus solfataricus DSM 1617 | Q97U21 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose + NADP+ | activity with substrate D-galactose in presence of cosubstrate NADP+ | Saccharolobus solfataricus | D-galactono-1,5-lactone + NADPH | - |
? | |
| D-galactose + NADP+ | activity with substrate D-galactose in presence of cosubstrate NADP+ | Saccharolobus solfataricus DSM 1617 | D-galactono-1,5-lactone + NADPH | - |
? | |
| D-glucose + NAD+ | - |
Saccharolobus solfataricus | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
| D-glucose + NAD+ | - |
Saccharolobus solfataricus DSM 1617 | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
| D-xylose + NADP+ | activity with substrate D-galactose in presence of cosubstrate NADP+ | Saccharolobus solfataricus | D-xylono-1,5-lactone + NADPH + H+ | - |
? | |
| D-xylose + NADP+ | activity with substrate D-galactose in presence of cosubstrate NADP+ | Saccharolobus solfataricus DSM 1617 | D-xylono-1,5-lactone + NADPH + H+ | - |
? | |
| additional information | no substrates: D-allose, D-mannose, L-arabinose, D-ribose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose, ethanol | Saccharolobus solfataricus | ? | - |
? | |
| additional information | no substrates: D-allose, D-mannose, L-arabinose, D-ribose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose, ethanol | Saccharolobus solfataricus DSM 1617 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 39000, SDS-PAGE, x * 39970, calculated | Saccharolobus solfataricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glucose 1-dehydrogenase 3 | - |
Saccharolobus solfataricus |
| SSO3204 | - |
Saccharolobus solfataricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 19.34 | - |
D-glucose | cosubstrate NADP+, 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 26.74 | - |
NADP+ | cosubstrate D-glucose, 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 28.52 | - |
NADP+ | cosubstrate D-glucose, 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 44.17 | - |
NADP+ | cosubstrate D-glucose, 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 54.47 | - |
NAD+ | cosubstrate D-glucose, 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 68.23 | - |
D-glucose | cosubstrate NAD+, 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 70.49 | - |
NAD+ | cosubstrate D-glucose, 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 105.4 | - |
NAD+ | cosubstrate D-glucose, 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Saccharolobus solfataricus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.74 | - |
NAD+ | cosubstrate D-glucose, 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 14.86 | - |
D-glucose | cosubstrate NAD+, 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 31.6 | - |
NAD+ | cosubstrate D-glucose, 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 31.95 | - |
NAD+ | cosubstrate D-glucose, 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 115.2 | - |
D-glucose | cosubstrate NADP+, 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 178.3 | - |
NADP+ | cosubstrate D-glucose, 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 203.7 | - |
NADP+ | cosubstrate D-glucose, 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 294.5 | - |
NADP+ | cosubstrate D-glucose, 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
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