Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.47 extracted from

  • Ohshima, T.; Ito, Y.; Sakuraba, H.; Goda, S.; Kawarabayasi, Y.
    The Sulfolobus tokodaii gene ST1704 codes highly thermostable glucose dehydrogenase (2003), J. Mol. Catal. B, 23, 281-289.
No PubMed abstract available
Search for more literature for 1.1.1.47

Application

Application Comment Organism
analysis enzyme can be used for glucose determination Sulfurisphaera tokodaii
biotechnology larger scale production of NAD(P)H in bioreactors by usage of the enzyme, a thermostable enzyme is advantageous Sulfurisphaera tokodaii
diagnostics enzyme can be used for glucose determination Sulfurisphaera tokodaii

Cloned(Commentary)

Cloned (Comment) Organism
gene ST1704, expression in enzyme-deficient Escherichia coli strain Sulfurisphaera tokodaii

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 1 mM, 33% inhibition Sulfurisphaera tokodaii
Co2+ 1 mM, 44% inhibition Sulfurisphaera tokodaii
Cu2+ 1 mM, 83% inhibition Sulfurisphaera tokodaii
EDTA 1 mM, 68% inhibition, fully reversible by 20 mM MgCl2 Sulfurisphaera tokodaii
Hg2+ 1 mM, 85% inhibition Sulfurisphaera tokodaii
Mn2+ 1 mM, 41% inhibition Sulfurisphaera tokodaii
Ni2+ 1 mM, 85% inhibition Sulfurisphaera tokodaii
p-chloromercuribenzoate 1 mM, 93% inhibition Sulfurisphaera tokodaii
Zn2+ 1 mM, 25% inhibition Sulfurisphaera tokodaii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetic analysis Sulfurisphaera tokodaii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates by 122% at 1 mM Sulfurisphaera tokodaii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 4 * 41000, recombinant enzyme, SDS-PAGE Sulfurisphaera tokodaii
160000
-
 recombinant enzyme, gel filtration Sulfurisphaera tokodaii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-D-glucose + NAD(P)+ Sulfurisphaera tokodaii
-
 D-glucono-1,5-lactone + NAD(P)H + H+
-
 ?
beta-D-glucose + NAD(P)+ Sulfurisphaera tokodaii 7
-
 D-glucono-1,5-lactone + NAD(P)H + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii
-
 gene ST1704
-
Sulfurisphaera tokodaii 7
-
 gene ST1704
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli by heat treament and 2 chromatographical steps Sulfurisphaera tokodaii

Reaction

Reaction Comment Organism Reaction ID
D-glucose + NAD(P)+ = D-glucono-1,5-lactone + NAD(P)H + H+ sequential ordered bi-bi mechanism Sulfurisphaera tokodaii
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
32
-
 purified recombinant enzyme Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-2-deoxy-D-glucose + NAD(P)+
-
 Sulfurisphaera tokodaii ?
-
 ?
2-amino-2-deoxy-D-glucose + NAD(P)+
-
 Sulfurisphaera tokodaii 7 ?
-
 ?
2-deoxy-D-glucose + NAD(P)+ low activity Sulfurisphaera tokodaii ?
-
 ?
2-deoxy-D-glucose + NAD(P)+ low activity Sulfurisphaera tokodaii 7 ?
-
 ?
6-deoxy-D-glucose + NAD(P)+
-
 Sulfurisphaera tokodaii ?
-
 ?
beta-D-galactose + NADP+ low activity Sulfurisphaera tokodaii D-galactono-1,5-lactone + NADPH
-
 ?
beta-D-glucose + NAD(P)+
-
 Sulfurisphaera tokodaii D-glucono-1,5-lactone + NAD(P)H + H+
-
 ?
beta-D-glucose + NAD(P)+
-
 Sulfurisphaera tokodaii 7 D-glucono-1,5-lactone + NAD(P)H + H+
-
 ?
D-allose + NAD(P)+ low activity Sulfurisphaera tokodaii ?
-
 ?
D-altrose + NADP+ low activity Sulfurisphaera tokodaii ?
-
 ?
D-gulose + NAD(P)+ low activity Sulfurisphaera tokodaii ?
-
 ?
D-ribose + NADP+ low activity Sulfurisphaera tokodaii ?
-
 ?
D-ribose + NADP+ low activity Sulfurisphaera tokodaii 7 ?
-
 ?
D-xylose + NAD(P)+
-
 Sulfurisphaera tokodaii ?
-
 ?
additional information broad substrate specificity, no activity with D-mannose, D-ribose, and glucose 6-phosphate, cofactor specificity depends on the sugar sustrate Sulfurisphaera tokodaii ?
-
 ?
additional information broad substrate specificity, no activity with D-mannose, D-ribose, and glucose 6-phosphate, cofactor specificity depends on the sugar sustrate Sulfurisphaera tokodaii 7 ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 41000, recombinant enzyme, SDS-PAGE Sulfurisphaera tokodaii

Synonyms

Synonyms Comment Organism
GDH
-
 Sulfurisphaera tokodaii
glucose dehydrogenase
-
 Sulfurisphaera tokodaii
NAD(P)-dependent glucose-1-dehydrogenase
-
 Sulfurisphaera tokodaii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
 Sulfurisphaera tokodaii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 highly thermostable enzyme Sulfurisphaera tokodaii
80
-
 15 min, more than 90% remaining activity, pH 8.0 Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Sulfurisphaera tokodaii

pH Range

pH Minimum pH Maximum Comment Organism
4.5 11 50% of maximal activity at pH 4.5 and pH 11.0 Sulfurisphaera tokodaii

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10.5 stable at 37°C for 30 min Sulfurisphaera tokodaii

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Sulfurisphaera tokodaii
NADH
-
 Sulfurisphaera tokodaii
NADP+
-
 Sulfurisphaera tokodaii
NADPH
-
 Sulfurisphaera tokodaii