General Stability | Organism |
---|---|
purified enzyme is indefinitely stable in the frozen state or in solution at pH 6.5 containing 3 M NaCl and a protein concentration of more than 0.5 mg/ml. Diluted enzyme solutions can be stabilized to the same degree by adding 0.5% polyvinylpyrrolidone. Any reduction of the ionic strength of enzyme solution leads to an irreversible inactivation which can be only partially prevented by additrion of polyvinylpyrrolidone | Priestia megaterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.5 | - |
NAD+ | pH 9.0, 25°C | Priestia megaterium | |
47.5 | - |
beta-D-glucose | pH 9.0, 25°C | Priestia megaterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
4 * 30000, SDS-PAGE | Priestia megaterium |
116000 | - |
gel filtration | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
hydroxyapatite, QAE-Sephadex | Priestia megaterium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
550 | - |
- |
Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-2-deoxy-D-glucose + NAD+ | 14% of the activity with D-glucose | Priestia megaterium | 2-amino-2-deoxy-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
2-deoxy-D-glucose + NAD+ | 114% of the activity with D-glucose | Priestia megaterium | 2-deoxy-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucose + NAD+ | the enzyme is highly specific for beta-D-glucose | Priestia megaterium | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucose + NADP+ | the enzyme is highly specific for beta-D-glucose | Priestia megaterium | D-glucono-1,5-lactone + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 30000, SDS-PAGE | Priestia megaterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
sharp decrease above, Tris-HCl buffer | Priestia megaterium |
9 | - |
acetate/borate buffer | Priestia megaterium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Priestia megaterium | |
NADP+ | - |
Priestia megaterium |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Priestia megaterium | isoelectric focusing, two protein bands: the major one is located at pH 6.0 and a very weak one is located at pH 4.7. After preincubation in 8 M urea, only the major band at pH 6.0 can be observed | 4.8 | 4.7 |
Priestia megaterium | isoelectric focusing, two protein bands: the major one is located at pH 6.0 and a very weak one is located at pH 4.7. After preincubation in 8 M urea, only the major band at pH 6.0 can be observed | - |
6 |