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Literature summary for 1.1.1.47 extracted from

  • Campbell, D.P.; Carper, W.R.; Thompson, R.E.
    Bovine liver glucose dehydrogenase: isolation and characterization (1982), Arch. Biochem. Biophys., 215, 289-301.
    View publication on PubMed
Search for more literature for 1.1.1.47

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0212
-
 NAD+ pH 9.5, 30°C, substrate beta-D-glucose Bos taurus
0.0255
-
 D-glucose 6-phosphate coenzyme NADP+ Bos taurus
0.062
-
 NADP+ pH 9.5, 30°C, reaction with D-glucose 6-phosphate Bos taurus
3.38
-
 beta-D-glucose pH 9.5, 30°C, coenzyme NAD+ Bos taurus
47.22
-
 beta-D-glucose pH 9.5, 30°C, coenzyme NADP+ Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Bos taurus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
 4 * 59000, SDS-PAGE Bos taurus
235000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-D-glucose + NAD(P)+ Bos taurus multifunctional enzyme is involved in several catabolic sugar pathways in the endoplasmic reticulum D-glucono-1,5-lactone + NAD(P)H + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
lipoprotein the lipid component may be involved in the molecular structure and function of the enzyme Bos taurus

Purification (Commentary)

Purification (Comment) Organism
Triton X-100, acid treatment, ammonium sulfate, Sephacryl S-200, Sephacryl S-300, DEAE-cellulose, Sephacryl S-300 Bos taurus

Reaction

Reaction Comment Organism Reaction ID
D-glucose + NAD(P)+ = D-glucono-1,5-lactone + NAD(P)H + H+ ordered bi bi mechanism Bos taurus
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.25
-
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucose + NAD(P)+ multifunctional enzyme is involved in several catabolic sugar pathways in the endoplasmic reticulum Bos taurus D-glucono-1,5-lactone + NAD(P)H + H+
-
 ?
beta-D-glucose + NAD+
-
 Bos taurus D-glucono-1,5-lactone + NADH + H+
-
 ?
D-glucose 6-phosphate + NADP+
-
 Bos taurus D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 59000, SDS-PAGE Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
 Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
7.5 10.5 at pH 7.5 and 10.0 respectively 75% and 50% activity compared to optimal pH Bos taurus