Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.47 extracted from

  • Giardina, P.; DeBasia, M.G.; DeRosa, M.; Gambacort, A.; Buonocore, V.
    Glucose dehydrogenase from the thermoacidophilic archaebacterium Sulfolobus solfataricus (1986), Biochem. J., 239, 517-522.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.47

General Stability

General Stability Organism
0.05% SDS, no loss of activity, 0.1%, slow decrease Saccharolobus solfataricus
2 M urea, 24 h, room temperature, no loss of activity, slow decrease at 4 M Saccharolobus solfataricus
4 M guanidinium chloride, rapid inactivation Saccharolobus solfataricus
H4-furan, rapid inactivation Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 1 mM, room temperature, pH 9.0, rapid inactivation Saccharolobus solfataricus
5,5'-dithiobis(2-nitrobenzoic acid) 1 mM, room temperature, pH 9.0, 50% inactivation Saccharolobus solfataricus
EDTA 50 mM, 5% activity left Saccharolobus solfataricus
N-ethylmaleimide 3 mM, room temperature, pH 9.0, 50% inactivation Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 NADP+ pH 9.0, 70°C Saccharolobus solfataricus
0.44
-
 beta-D-glucose pH 9.0, 70°C, coenzyme NADP+ Saccharolobus solfataricus
1.2
-
 NAD+ pH 9.0, 70°C Saccharolobus solfataricus
2.2
-
 beta-D-glucose pH 9.0, 70°C, coenzyme NADP+ Saccharolobus solfataricus
8
-
 beta-D-glucose pH 9.0, 70°C, coenzyme NAD+ Saccharolobus solfataricus
22
-
 beta-D-galactose pH 9.0, 70°C, coenzyme NADP+ Saccharolobus solfataricus
68
-
 beta-D-xylose pH 9.0, 70°C, coenzyme NAD+ Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ maximal activity at 20 mM Saccharolobus solfataricus
Mg2+ maximal activity at 20 mM Saccharolobus solfataricus
Mn2+ maximal activity at 20 mM Saccharolobus solfataricus
Zn2+
-
 Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 4 * 33000, SDS-PAGE Saccharolobus solfataricus
124000
-
 gel filtration Saccharolobus solfataricus
130000
-
 sedimentation equilibrium Saccharolobus solfataricus

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 50%, 24 h, room temperature, no loss of activity Saccharolobus solfataricus
Ethanol 100%, slow decrease Saccharolobus solfataricus
Methanol 50%, 24 h, room temperature, no loss of activity Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, SP-Sephadex, Phenyl-Sepharose, Affi-Gel Blue Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
437
-
-
 Saccharolobus solfataricus

Storage Stability

Storage Stability Organism
4°C, 20 mM MgCl2, 20% ethylene glycol, several months, stable Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-2-deoxy-D-glucose + NAD+ 26% of the activity with D-glucose Saccharolobus solfataricus 2-amino-2-deoxy-D-glucono-1,5-lactone + NADH + H+
-
 ?
2-amino-2-deoxy-D-glucose + NADP+ 5% of the activity with D-glucose and NAD+ Saccharolobus solfataricus 2-amino-2-deoxy-D-glucono-1,5-lactone + NADPH + H+
-
 ?
2-deoxy-D-glucose + NAD+ 12% of the activity with glucose Saccharolobus solfataricus 2-deoxy-D-glucono-1,5-lactone + NADH + H+
-
 ?
2-deoxy-D-glucose + NADP+ 25% of the activity with D-glucose and NAD+ Saccharolobus solfataricus 2-deoxy-D-glucono-1,5-lactone + NADPH + H+
-
 ?
6-deoxy-D-glucose + NAD+ 66% of the activity with D-glucose Saccharolobus solfataricus 6-deoxy-D-glucono-1,5-lactone + NADH + H+
-
 ?
6-deoxy-D-glucose + NADP+ 9% of the activity with D-glucose and NAD+ Saccharolobus solfataricus ? + NADPH
-
 ?
beta-D-glucose + NAD+ 9% of the activity with NAD+ Saccharolobus solfataricus D-glucono-1,5-lactone + NADH + H+
-
 ?
beta-D-glucose + NADP+
-
 Saccharolobus solfataricus D-glucono-1,5-lactone + NADPH + H+
-
 ?
beta-D-xylose + NAD+ 26% activity compared to D-glucose Saccharolobus solfataricus D-xylono-1,5-lactone + NADH + H+
-
 r
D-allose + NAD+ 8% of the activity with D-glucose Saccharolobus solfataricus D-allono-1,5-lactone + NADH
-
 ?
D-allose + NADP+ 13% of the activity with D-glucose and NAD+ Saccharolobus solfataricus D-allono-1,5-lactone + NADPH
-
 ?
D-altrose + NAD+ 5% of the activity with D-glucose Saccharolobus solfataricus D-altrono-1,5-lactone + NADH
-
 ?
D-altrose + NADP+ 12% of the activity with D-glucose and NAD+ Saccharolobus solfataricus D-altrono-1,5-lactone + NADPH
-
 ?
D-galactose + NAD+ 15% of the activity with D-glucose and NAD+ Saccharolobus solfataricus D-galactono-1,5-lactone + NADH
-
 ?
D-gulose + NAD+ 8% of the activity with D-glucose Saccharolobus solfataricus D-gulono-1,5-lactone + NADH
-
 ?
D-gulose + NADP+ 12% of the activity with D-glucose and NAD+ Saccharolobus solfataricus D-gulono-1,5-lactone + NADPH
-
 ?
D-idose + NAD+ 65% of the activity with D-glucose Saccharolobus solfataricus D-idono-1,5-lactone + NADH
-
 ?
D-idose + NADP+ 12% of the activity with D-glucose and NAD+ Saccharolobus solfataricus D-idono-1,5-lactone + NADPH
-
 ?
D-mannose + NADP+ no activity Saccharolobus solfataricus D-mannono-1,5-lactone + NADPH
-
 ?
D-ribose + NADP+ 4% of the activity with D-glucose and NADP+ Saccharolobus solfataricus D-ribono-1,5-lactone + NADPH
-
 ?
D-xylose + NAD+ 28% of the activity with D-glucose and NAD+ Saccharolobus solfataricus D-xylono-1,5-lactone + NADH + H+
-
 ?
D-xylose + NADP+ 28% of the activity with D-glucose and NAD+ Saccharolobus solfataricus D-xylono-1,5-lactone + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 33000, SDS-PAGE Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
77
-
 at pH 8.0 Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 77 37°C: 20% of maximal activity, 77°C: optimum Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Saccharolobus solfataricus
NADP+
-
 Saccharolobus solfataricus