Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucono-1,5-lactone | forward reaction, oxidation, product inhibition, noncompetitive vs. beta-D-glucose and NADP+ | Halobacterium salinarum | |
NADP+ | reverse reaction, reduction, product inhibition, competitive vs. NADPH and noncompetitive vs. D-glucono-1-5-lactone | Halobacterium salinarum | |
NADPH | product inhibition, competitive with respect to NADP | Halobacterium salinarum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
beta-D-glucose | reduction, reverse reaction | Halobacterium salinarum | |
0.19 | - |
NADP+ | - |
Halobacterium salinarum | |
0.49 | - |
NADPH | - |
Halobacterium salinarum | |
2.57 | - |
beta-D-glucose | oxidation, forward reaction | Halobacterium salinarum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halobacterium salinarum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Sepharose 6B, DEAE-Sepharose, hydroxyapatite chromatography | Halobacterium salinarum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-glucose + NAD(P)+ = D-glucono-1,5-lactone + NAD(P)H + H+ | ordered bi bi mechanism, formation of ternary complexes, NADP+ adds first, NADPH formed dissociates last, for reverse reaction, NADPH adds first, NADP+ leaves last | Halobacterium salinarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucose + NADP+ | - |
Halobacterium salinarum | D-glucono-1,5-lactone + NADPH + H+ | - |
r |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Halobacterium salinarum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Halobacterium salinarum |